Recombinant Human Hsp47 protein is a Human Full Length protein, in the 19 to 418 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A E V K K P A A A A A P G T A E K L S P K A A T L A E R S A G L A F S L Y Q A M A K D Q A V E N I L V S P V V V A S S L G L V S L G G K A T T A S Q A K A V L S A E Q L R D E E V H A G L G E L L R S L S N S T A R N V T W K L G S R L Y G P S S V S F A D D F V R S S K Q H Y N C E H S K I N F R D K R S A L Q S I N E W A A Q T T D G K L P E V T K D V E R T D G A L L V N A M F F K P H W D E K F H H K M V D N R G F M V T R S Y T V G V M M M H R T G L Y N Y Y D D E K E K L Q I V E M P L A H K L S S L I I L M P H H V E P L E R L E K L L T K E Q L K I W M G K M Q K K A V A I S L P K G V V E V T H D L Q K H L A G L G L T E A I D K N K A D L S R M S G K K D L Y L A S V F H A T A F E L D T D G N P F D Q D I Y G R E E L R S P K L F Y A D H P F I F L V R D T Q S G S L L F I G R L V R P K G D K M R D E L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Alternative names
CBP1, CBP2, HSP47, SERPINH2, PIG14, SERPINH1, Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Rheumatoid arthritis-related antigen RA-A47, AsTP3, Colligin
Recommended products
Recombinant Human Hsp47 protein is a Human Full Length protein, in the 19 to 418 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P50454-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- A E V K K P A A A A A P G T A E K L S P K A A T L A E R S A G L A F S L Y Q A M A K D Q A V E N I L V S P V V V A S S L G L V S L G G K A T T A S Q A K A V L S A E Q L R D E E V H A G L G E L L R S L S N S T A R N V T W K L G S R L Y G P S S V S F A D D F V R S S K Q H Y N C E H S K I N F R D K R S A L Q S I N E W A A Q T T D G K L P E V T K D V E R T D G A L L V N A M F F K P H W D E K F H H K M V D N R G F M V T R S Y T V G V M M M H R T G L Y N Y Y D D E K E K L Q I V E M P L A H K L S S L I I L M P H H V E P L E R L E K L L T K E Q L K I W M G K M Q K K A V A I S L P K G V V E V T H D L Q K H L A G L G L T E A I D K N K A D L S R M S G K K D L Y L A S V F H A T A F E L D T D G N P F D Q D I Y G R E E L R S P K L F Y A D H P F I F L V R D T Q S G S L L F I G R L V R P K G D K M R D E L
- Accession
- P50454
- Protein length
- Full Length
- Predicted molecular weight
- 45.5 kDa
- Amino acids
- 19 to 418
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
- Sequence similarities
Belongs to the serpin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is stable after storage at:
• -20°C to -70°C for 12 months in lyophilized state;
• -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp47 also known as the heat shock protein 47 is a molecular chaperone with a molecular weight of approximately 47 kDa. It mainly resides in the endoplasmic reticulum and participates in collagen synthesis. Hsp47 binds specifically to unfolded procollagen chains ensures their proper folding and prevents premature aggregation. Due to its specialized function Hsp47 is highly expressed in collagen-producing cells like fibroblasts. Researchers commonly study this protein using techniques like Hsp47 ELISA to measure its expression or activity levels.
- Biological function summary
Hsp47 ensures the quality control of collagen within the secretory pathway. It does not form part of any large protein complexes but operates closely with collagen molecules. By stabilizing procollagen's triple-helix structure Hsp47 plays an important role in collagen biosynthesis. Dysfunctional collagen processes can arise without this protein's presence potentially leading to cellular stress or structural weaknesses within tissues rich in collagen.
- Pathways
Hsp47's activity integrates into the collagen synthesis and secretion pathways. This protein is related to other molecular chaperones such as Hsp70 that assist in maintaining protein homeostasis within the cell. Hsp47’s function links to fibrosis pathways because of its role in collagen maturation. In terms of fibrotic pathologies this involvement makes the chaperoning of procollagen a critical control point.
- Associated diseases and disorders
Disrupted Hsp47 function frequently associates with fibrotic conditions and osteogenesis imperfecta. Fibrosis results from excessive collagen deposition in which Hsp47 is deeply implicated. Additionally certain cancer types have shown altered Hsp47 expression correlating with increased cancer cell invasiveness due to extracellular matrix remodeling. Studies have noted interactions between Hsp47 and TGF-beta signaling in fibrotic tissues reinforcing its role in pathological collagen turnover.
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1 product image
SDS-PAGE - Recombinant Human Hsp47 protein (ab182711)
DTT-reduced (+) SDS-PAGE analysis of ab182711, staining overnight with Coomassie Blue. The protein migrates as 47 kDa under reducing condition due to glycosylation.
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