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AB95318

Recombinant Human HSPC150/UBE2T protein (His tag C-Terminus)

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Recombinant Human HSPC150/UBE2T protein (His tag C-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

HSPC150, PIG50, UBE2T, Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, E2 ubiquitin-conjugating enzyme T, Ubiquitin carrier protein T, Ubiquitin-protein ligase T

1 Images
SDS-PAGE - Recombinant Human HSPC150/UBE2T protein (His tag C-Terminus) (AB95318)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human HSPC150/UBE2T protein (His tag C-Terminus) (AB95318)

ab95318 at 3 μg on an SDS-PAGE gel (15%).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NPD8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as HSPC150
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NPD8","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPC150 also known as UBE2T is a protein involved in the ubiquitination process. It functions as a ubiquitin-conjugating enzyme essential for tagging proteins with ubiquitin marking them for degradation via the proteasome. UBE2T has a mass of approximately 22 kDa and shows expression in various tissues with higher levels found in the testis and bone marrow. It is critical in maintaining protein homeostasis by regulating the turnover of proteins within cells.
Biological function summary

HSPC150/UBE2T interacts with the Fanconi anemia (FA) complex playing a significant role within DNA repair processes. Its activity is necessary for mono-ubiquitination of the FANCD2 protein a central component of the FA pathway. Through this engagement UBE2T contributes to the repair of interstrand crosslinks in DNA therefore supporting the maintenance of genomic integrity. The protein's involvement in these processes highlights its importance in preserving proper cellular functions and preventing genomic instability.

Pathways

HSPC150/UBE2T is mainly involved in the DNA damage repair pathways particularly the Fanconi anemia pathway. In this context it collaborates with proteins like FANCL and RAD51 which are key players in homologous recombination and DNA repair. Ubiquitination of FANCD2 by UBE2T integrates into these pathways facilitating efficient DNA repair and ensuring cell survival following genotoxic stress.

Dysfunction of HSPC150/UBE2T links to cancer particularly with certain hematological malignancies. Alterations or overexpression of UBE2T can lead to defective DNA repair contributing to carcinogenesis due to accumulation of DNA damage. Furthermore associations with Fanconi anemia also illustrate its vital role given that mutations affecting UBE2T can hinder the pathway's function leading to bone marrow failure and increased cancer risk. The interaction with FANCD2 emphasizes its potential impact in conditions associated with genomic instability.
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Specifications

Form

Liquid

Additional notes

ab95318 is purified using conventional chromatography techniques.

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General info

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 19589784, PubMed : 28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed : 19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed : 20061386).

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Post-translational modifications

Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity (PubMed:16916645). In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity (PubMed:19111657).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 19589784, PubMed : 28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed : 19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed : 20061386).
See full target information UBE2T
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