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AB314620

Recombinant Human HTR2B Protein (His Tag)

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Recombinant Human HTR2B Protein (His Tag) is a Human Full Length protein, in the 1 to 481 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

5-hydroxytryptamine receptor 2B, 5-HT-2B, 5-HT2B, Serotonin receptor 2B, HTR2B

1 Images
SDS-PAGE - Recombinant Human HTR2B Protein (His Tag) (AB314620)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HTR2B Protein (His Tag) (AB314620)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Observed band size : 70.3 kDa, consistent with the theoretical size.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P41595

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose

storage-buffer

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Complementary Products

Biochemicals

AB286948

S1RA (E-52862)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MALSYRVSELQSTIPEHILQSTFVHVISSNWSGLQTESIPEEMKQIVEEQGNKLHWAALLILMVIIPTIGGNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLPLVLCPAWLFLDVLFSTASIMHLCAISVDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIETDVDNPNNITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYLVKNKPPQRLTWLTVSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGDETLMRRTSTIGKKSVQTISNEQRASKVLGIVFFLFLLMWCPFFITNITLVLCDSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDAFGRYITCNYRATKSVKTLRKRSSKIYFRNPMAENSKFFKKHGIRNGINPAMYQSPMRLRSSTIQSSSIILLDTLLLTENEGDKTEEQVSYV","proteinLength":"Full Length","predictedMolecularWeight":"70.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":481,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P41595","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

5-HT-2B also known as serotonin receptor 2B is a G-protein-coupled receptor that binds to serotonin. This protein with a molecular mass of approximately 55 kDa is located on the cell surface and exhibits a wide expression in tissues such as the heart lung liver and gastrointestinal tract. It contains several functional domains contributing to its activity mediating cellular responses through signaling cascades when serotonin binds to it.
Biological function summary

5-HT-2B plays a role in modulating the contractility and remodeling of cardiac tissue influencing vascular function and smooth muscle behavior impacting gastrointestinal motility and contributing to liver regeneration. It is not part of a large multiprotein complex but interacts with other cellular components to exert its biological functions. The receptor's activation leads to various intracellular responses influencing different physiological effects depending on the tissue context.

Pathways

Serotonin receptor 2B is involved in the serotonin signaling pathway and the calcium signaling pathway. These pathways impact several physiological processes including mood regulation and cardiovascular function. The receptor interacts with proteins such as phospholipase C which mediates the hydrolysis of phospholipids in the membrane releasing inositol trisphosphate and causing a rise in intracellular calcium levels important for function continuation.

Abnormalities in 5-HT-2B have been linked to cardiac valvulopathy and pulmonary hypertension. These conditions highlight the importance of its role in vascular and cardiac function. The receptor's dysregulation interacts with proteins such as endothelin-1 in vascular abnormalities and contributes to disease progression highlighting its potential as a target for therapeutic interventions in these disorders.
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Specifications

Form

Lyophilized

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General info

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 18703043, PubMed : 23519210, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed : 12970106, PubMed : 18703043, PubMed : 23519210, PubMed : 23519215, PubMed : 24357322, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 23519215, PubMed : 28129538, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). HTR2B is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain (By similarity). Plays a role in the regulation of behavior, including impulsive behavior (PubMed : 21179162). Required for normal proliferation of embryonic cardiac myocytes and normal heart development (By similarity). Protects cardiomyocytes against apoptosis (By similarity). Plays a role in the adaptation of pulmonary arteries to chronic hypoxia (By similarity). Plays a role in vasoconstriction (By similarity). Required for normal osteoblast function and proliferation, and for maintaining normal bone density (By similarity). Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity).

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

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Product protocols

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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 18703043, PubMed : 23519210, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed : 12970106, PubMed : 18703043, PubMed : 23519210, PubMed : 23519215, PubMed : 24357322, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 7926008, PubMed : 8078486, PubMed : 8143856). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 23519215, PubMed : 28129538, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). HTR2B is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581, PubMed : 8078486, PubMed : 8143856, PubMed : 8882600). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 23519215, PubMed : 28129538, PubMed : 30127358, PubMed : 36087581). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain (By similarity). Plays a role in the regulation of behavior, including impulsive behavior (PubMed : 21179162). Required for normal proliferation of embryonic cardiac myocytes and normal heart development (By similarity). Protects cardiomyocytes against apoptosis (By similarity). Plays a role in the adaptation of pulmonary arteries to chronic hypoxia (By similarity). Plays a role in vasoconstriction (By similarity). Required for normal osteoblast function and proliferation, and for maintaining normal bone density (By similarity). Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity).
See full target information HTR2B
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