Recombinant Human HTR7 Protein (His Tag) is a Human Full Length protein, in the 1 to 479 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- 10x His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M M D V N S S G R P D L Y G H L R S F L L P E V G R G L P D L S P D G G A D P V A G S W A P H L L S E V T A S P A P T W D A P P D N A S G C G E Q I N Y G R V E K V V I G S I L T L I T L L T I A G N C L V V I S V C F V K K L R Q P S N Y L I V S L A L A D L S V A V A V M P F V S V T D L I G G K W I F G H F F C N V F I A M D V M C C T A S I M T L C V I S I D R Y L G I T R P L T Y P V R Q N G K C M A K M I L S V W L L S A S I T L P P L F G W A Q N V N D D K V C L I S Q D F G Y T I Y S T A V A F Y I P M S V M L F M Y Y Q I Y K A A R K S A A K H K F P G F P R V E P D S V I A L N G I V K L Q K E V E E C A N L S R L L K H E R K N I S I F K R E Q K A A T T L G I I V G A F T V C W L P F F L L S T A R P F I C G T S C S C I P L W V E R T F L W L G Y A N S L I N P F I Y A F F N R D L R T T Y R S L L Q C Q Y R N I N R K L S A A G M H E A L K L A E R P E R P E F V L R A C T R R V L L R P E K R P P V S V W V L Q S P D H H N W L A D K M L T T V E K K V M I H D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed:35714614, PubMed:8226867). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:35714614, PubMed:8226867). HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed:35714614).
Alternative names
5-hydroxytryptamine receptor 7, 5-HT-7, 5-HT7, 5-HT-X, Serotonin receptor 7, HTR7
Recommended products
Recombinant Human HTR7 Protein (His Tag) is a Human Full Length protein, in the 1 to 479 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- 10x His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P34969-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.14% PBS
Sequence info
Amino acid sequence
- M M D V N S S G R P D L Y G H L R S F L L P E V G R G L P D L S P D G G A D P V A G S W A P H L L S E V T A S P A P T W D A P P D N A S G C G E Q I N Y G R V E K V V I G S I L T L I T L L T I A G N C L V V I S V C F V K K L R Q P S N Y L I V S L A L A D L S V A V A V M P F V S V T D L I G G K W I F G H F F C N V F I A M D V M C C T A S I M T L C V I S I D R Y L G I T R P L T Y P V R Q N G K C M A K M I L S V W L L S A S I T L P P L F G W A Q N V N D D K V C L I S Q D F G Y T I Y S T A V A F Y I P M S V M L F M Y Y Q I Y K A A R K S A A K H K F P G F P R V E P D S V I A L N G I V K L Q K E V E E C A N L S R L L K H E R K N I S I F K R E Q K A A T T L G I I V G A F T V C W L P F F L L S T A R P F I C G T S C S C I P L W V E R T F L W L G Y A N S L I N P F I Y A F F N R D L R T T Y R S L L Q C Q Y R N I N R K L S A A G M H E A L K L A E R P E R P E F V L R A C T R R V L L R P E K R P P V S V W V L Q S P D H H N W L A D K M L T T V E K K V M I H D
- Accession
- P34969
- Protein length
- Full Length
- Actual molecular weight
- 56.4 kDa
- Amino acids
- 1 to 479
- Nature
- Recombinant
- Tags
- 10x His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen (PubMed:35714614, PubMed:8226867). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:35714614, PubMed:8226867). HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed:35714614).
- Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- The product can be stored for up to 12 months
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 5-HT7 receptor also known as the serotonin receptor 5-HT7 is a G-protein-coupled receptor that plays a mechanical role in neurotransmission. This receptor belongs to the serotonin receptor family and typically weighs around 46 kDa. You find the 5-HT7 receptor expressed in various tissues including the brain particularly in regions such as the thalamus hypothalamus and hippocampus as well as in smooth muscles and the gastrointestinal tract. Its function is mediated through Gs proteins which activate adenylate cyclase leading to increased levels of cyclic adenosine monophosphate (cAMP).
- Biological function summary
The 5-HT7 receptor contributes to multiple physiological processes including the regulation of circadian rhythms mood and cognitive functions. It operates as part of complex signaling networks within the central nervous system and other systems throughout the body. The receptor plays a significant role in modulating neurotransmitter release and neuronal excitability. Through these mechanisms it influences various behavioral and physiological outputs that are critical for the maintenance of homeostasis.
- Pathways
The 5-HT7 receptor is a pivotal component of serotoninergic signaling especially influencing the cAMP signaling cascade. This pathway interacts with proteins like adenylate cyclase and cAMP-dependent protein kinase impacting functions such as learning and memory. The receptor's modulation of cAMP levels places it within important pathways that affect cell signaling and metabolism including the broader serotonergic system which involves other serotonin receptors such as 5-HT1A and 5-HT2A.
- Associated diseases and disorders
Alterations in the function or expression of the 5-HT7 receptor are linked to a variety of conditions. It has been implicated in psychiatric disorders such as depression and schizophrenia where disruptions in serotonin signaling are common. In these contexts the receptor's interaction with other neurotransmitter systems including dopamine comes into play with proteins like the dopamine D2 receptor being relevant. The 5-HT7 receptor is also associated with migraine as it plays a role in the dilation of cerebral blood vessels and transmission of nociceptive signals making it a target for therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human HTR7 Protein (His Tag) (ab314621)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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