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AB268648

Recombinant Human htrA1 (mutated S328A) protein (His tag)

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Recombinant Human htrA1 (mutated S328A) protein (His tag) is a Human Fragment protein, in the 161 to 379 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

HTRA, PRSS11, HTRA1, Serine protease HTRA1, High-temperature requirement A serine peptidase 1, L56, Serine protease 11

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SDS-PAGE - Recombinant Human htrA1 (mutated S328A) protein (His tag) (AB268648)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human htrA1 (mutated S328A) protein (His tag) (AB268648)

SDS-PAGE analysis of ab268648.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q92743

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.79% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":379,"aminoAcidStart":161,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q92743","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HtrA1 also known as High Temperature Requirement A Serine Peptidase 1 functions mechanically as a serine protease. It has a mass of approximately 51 kDa and plays a critical role in protein quality control by degrading misfolded proteins. HtrA1 is expressed in various tissues including the placenta brain and lung. It localizes to the extracellular region indicating its involvement in extracellular matrix regulation.
Biological function summary

HtrA1 participates in cellular processes including protein degradation and modulation of signaling pathways. It does not have a known direct association with protein complexes but its protease activity affects extracellular matrix components and influences cellular homeostasis. HtrA1's regulatory function can affect cell growth and differentiation impacting tissue development and repair mechanisms.

Pathways

HtrA1 interacts significantly with the TGF-beta signaling pathway where its protease activity modulates the availability and activity of TGF-beta ligands and receptors. It also plays a role in the Wnt signaling pathway by influencing the stability of pathway-related proteins. HtrA1 interacts with proteins such as TGF-beta contributing to the regulation of extracellular matrix and cellular responses to external signals.

HtrA1 has connections to osteoarthritis and age-related macular degeneration (AMD). Elevated expression of HtrA1 correlates with the degeneration of articular cartilage and extracellular matrix breakdown in osteoarthritis. In AMD HtrA1 influences the structural integrity of ocular tissues with disturbances in this balance linked to progressive vision loss. Its activity interacts with proteins such as matrix metalloproteinases in these conditions contributing to the pathogenic processes.
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Specifications

Form

Liquid

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General info

Function

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

Sequence similarities

Belongs to the peptidase S1C family.

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Product protocols

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Target data

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
See full target information HTRA1 mutated S328A
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