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AB217828

Recombinant Human HtrA3 protein (His tag)

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Recombinant Human HtrA3 protein (His tag) is a Human Fragment protein, in the 132 to 353 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

PRSP, HTRA3, Serine protease HTRA3, High-temperature requirement factor A3, Pregnancy-related serine protease

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SDS-PAGE - Recombinant Human HtrA3 protein (His tag) (AB217828)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HtrA3 protein (His tag) (AB217828)

SDS-PAGE analysis of ab217828.

Key facts

Purity

>95% Densitometry

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P83110

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.79% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"QLSSPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIITNAHVVSSNSAAPGRQQLKVQLQNGDSYEATIKDIDKKSDIATIKIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIVSTAQREGRELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDRITRFLTEFQDKQIKDWK","proteinLength":"Fragment","predictedMolecularWeight":"27 kDa","actualMolecularWeight":null,"aminoAcidEnd":353,"aminoAcidStart":132,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P83110","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HtrA3 also known as high temperature requirement A3 is a serine protease that regulates protein quality control by degrading misfolded or damaged proteins. With a molecular mass of approximately 50 kDa HtrA3 is expressed in a variety of tissues including placenta lung and heart. Expression levels vary in different cell types which indicates a diverse role. The active form of HtrA3 often results from the removal of its N-terminal inhibitory domain allowing it to target specific substrates for degradation.
Biological function summary

HtrA3 plays a role in cellular stress response and apoptosis regulation. This protein engages in mitochondrial pathways to exert its pro-apoptotic effects. HtrA3 often functions independently rather than as part of a complex although its influence on pathways can involve interactions with other proteins pivotal to cell survival. In cellular stress conditions HtrA3 modulates the stability and function of key proteins involved in apoptosis.

Pathways

One critical interaction includes HtrA3's involvement in the modulation of the TGF-beta signaling pathway which plays a significant role in cell proliferation and differentiation. HtrA3 may interact with proteins such as Smad proteins within this pathway to influence cellular outcomes. Additionally HtrA3 can impact the oxidative stress response through its proteolytic activity affecting proteins involved in the reduction of oxidative damage.

Aberrant HtrA3 expression links to cancer and cardiovascular diseases. Reduced levels of HtrA3 have been observed in various cancers including ovarian cancer where low expression may correlate with poor prognosis. Moreover alterations in HtrA3 expression impact cardiovascular health potentially connecting with proteins like p53 which is important for the cellular stress responses in these disorders. Understanding HtrA3's roles can provide insight into its potential as a biomarker or therapeutic target in these disease contexts.
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Specifications

Form

Liquid

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General info

Function

Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed : 21321049, PubMed : 22229724).

Sequence similarities

Belongs to the peptidase S1C family.

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Product protocols

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Target data

Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed : 21321049, PubMed : 22229724).
See full target information HTRA3
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