Recombinant Human HuD protein (His tag) is a Human Full Length protein, in the 1 to 380 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
M V M I I S T M E P Q V S N G P T S N T S N G P S S N N R N C P S P M Q T G A T T D D S K T N L I V N Y L P Q N M T Q E E F R S L F G S I G E I E S C K L V R D K I T G Q S L G Y G F V N Y I D P K D A E K A I N T L N G L R L Q T K T I K V S Y A R P S S A S I R D A N L Y V S G L P K T M T Q K E L E Q L F S Q Y G R I I T S R I L V D Q V T G V S R G V G F I R F D K R I E A E E A I K G L N G Q K P S G A T E P I T V K F A N N P S Q K S S Q A L L S Q L Y Q S P N R R Y P G P L H H Q A Q R F R L D N L L N M A Y G V K R L M S G P V P P S A C P P R F S P I T I D G M T S L V G M N I P G H T G T G W C I F V Y N L S P D S D E S V L W Q L F G P F G A V N N V K V I R D F N T N K C K G F G F V T M T N Y D E A A M A I A S L N G Y R L G D R V L Q V S F K T N K A H K S
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs (PubMed:7898713, PubMed:10710437, PubMed:12034726, PubMed:12468554, PubMed:17035636, PubMed:17234598). Plays a role in the regulation of mRNA stability, alternative splicing and translation (PubMed:7898713, PubMed:10710437, PubMed:12034726, PubMed:12468554, PubMed:17035636, PubMed:17234598). Binds to AU-rich element (ARE) sequences in the 3' untranslated region (UTR) of target mRNAs, including GAP43, VEGF, FOS, CDKN1A and ACHE mRNA (PubMed:7898713, PubMed:10710437, PubMed:12034726, PubMed:12468554). Many of the target mRNAs are coding for RNA-binding proteins, transcription factors and proteins involved in RNA processing and/or neuronal development and function (By similarity). By binding to the mRNA 3'UTR, decreases mRNA deadenylation and thereby contributes to the stabilization of mRNA molecules and their protection from decay (PubMed:12034726). Also binds to the polyadenylated (poly(A)) tail in the 3'UTR of mRNA, thereby increasing its affinity for mRNA binding (PubMed:12034726). Mainly plays a role in neuron-specific RNA processing by stabilization of mRNAs such as GAP43, ACHE and mRNAs of other neuronal proteins, thereby contributing to the differentiation of neural progenitor cells, nervous system development, learning and memory mechanisms (PubMed:12034726, PubMed:12468554, PubMed:17234598, PubMed:18218628). Involved in the negative regulation of the proliferative activity of neuronal stem cells and in the positive regulation of neuronal differentiation of neural progenitor cells (By similarity). Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone of the hippocampus by binding to and stabilizing SATB1 mRNA (By similarity). Binds and stabilizes MSI1 mRNA in neural stem cells (By similarity). Exhibits increased binding to ACHE mRNA during neuronal differentiation, thereby stabilizing ACHE mRNA and enhancing its expression (PubMed:12468554, PubMed:17234598). Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). May bind to APP and BACE1 mRNAS and the BACE1AS lncRNA and enhance their stabilization (PubMed:24857657). Plays a role in neurite outgrowth and in the establishment and maturation of dendritic arbors, thereby contributing to neocortical and hippocampal circuitry function (By similarity). Stabilizes GAP43 mRNA and protects it from decay during postembryonic development in the brain (PubMed:12034726). By promoting the stabilization of GAP43 mRNA, plays a role in NGF-mediated neurite outgrowth (By similarity). Binds to BDNF long 3'UTR mRNA, thereby leading to its stabilization and increased dendritic translation after activation of PKC (By similarity). By increasing translation of BDNF after nerve injury, may contribute to nerve regeneration (By similarity). Acts as a stabilizing factor by binding to the 3'UTR of NOVA1 mRNA, thereby increasing its translation and enhancing its functional activity in neuron-specific splicing (PubMed:18218628). Stimulates translation of mRNA in a poly(A)- and cap-dependent manner, possibly by associating with the EIF4F cap-binding complex (By similarity). May also negatively regulate translation by binding to the 5'UTR of Ins2 mRNA, thereby repressing its translation (By similarity). Upon glucose stimulation, Ins2 mRNA is released from ELAVL4 and translational inhibition is abolished (By similarity). Also plays a role in the regulation of alternative splicing (PubMed:17035636). May regulate alternative splicing of CALCA pre-mRNA into Calcitonin and Calcitonin gene-related peptide 1 (CGRP) by competing with splicing regulator TIAR for binding to U-rich intronic sequences of CALCA pre-mRNA (PubMed:17035636).
ELAV-like protein 4, Hu-antigen D, Paraneoplastic encephalomyelitis antigen HuD, HuD, PNEM, HUD, ELAVL4
Recombinant Human HuD protein (His tag) is a Human Full Length protein, in the 1 to 380 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
No
Human
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
M V M I I S T M E P Q V S N G P T S N T S N G P S S N N R N C P S P M Q T G A T T D D S K T N L I V N Y L P Q N M T Q E E F R S L F G S I G E I E S C K L V R D K I T G Q S L G Y G F V N Y I D P K D A E K A I N T L N G L R L Q T K T I K V S Y A R P S S A S I R D A N L Y V S G L P K T M T Q K E L E Q L F S Q Y G R I I T S R I L V D Q V T G V S R G V G F I R F D K R I E A E E A I K G L N G Q K P S G A T E P I T V K F A N N P S Q K S S Q A L L S Q L Y Q S P N R R Y P G P L H H Q A Q R F R L D N L L N M A Y G V K R L M S G P V P P S A C P P R F S P I T I D G M T S L V G M N I P G H T G T G W C I F V Y N L S P D S D E S V L W Q L F G P F G A V N N V K V I R D F N T N K C K G F G F V T M T N Y D E A A M A I A S L N G Y R L G D R V L Q V S F K T N K A H K S
Full Length
61.8 kDa
1 to 380
Recombinant
His tag N-Terminus
Liquid
RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs (PubMed:7898713, PubMed:10710437, PubMed:12034726, PubMed:12468554, PubMed:17035636, PubMed:17234598). Plays a role in the regulation of mRNA stability, alternative splicing and translation (PubMed:7898713, PubMed:10710437, PubMed:12034726, PubMed:12468554, PubMed:17035636, PubMed:17234598). Binds to AU-rich element (ARE) sequences in the 3' untranslated region (UTR) of target mRNAs, including GAP43, VEGF, FOS, CDKN1A and ACHE mRNA (PubMed:7898713, PubMed:10710437, PubMed:12034726, PubMed:12468554). Many of the target mRNAs are coding for RNA-binding proteins, transcription factors and proteins involved in RNA processing and/or neuronal development and function (By similarity). By binding to the mRNA 3'UTR, decreases mRNA deadenylation and thereby contributes to the stabilization of mRNA molecules and their protection from decay (PubMed:12034726). Also binds to the polyadenylated (poly(A)) tail in the 3'UTR of mRNA, thereby increasing its affinity for mRNA binding (PubMed:12034726). Mainly plays a role in neuron-specific RNA processing by stabilization of mRNAs such as GAP43, ACHE and mRNAs of other neuronal proteins, thereby contributing to the differentiation of neural progenitor cells, nervous system development, learning and memory mechanisms (PubMed:12034726, PubMed:12468554, PubMed:17234598, PubMed:18218628). Involved in the negative regulation of the proliferative activity of neuronal stem cells and in the positive regulation of neuronal differentiation of neural progenitor cells (By similarity). Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone of the hippocampus by binding to and stabilizing SATB1 mRNA (By similarity). Binds and stabilizes MSI1 mRNA in neural stem cells (By similarity). Exhibits increased binding to ACHE mRNA during neuronal differentiation, thereby stabilizing ACHE mRNA and enhancing its expression (PubMed:12468554, PubMed:17234598). Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). May bind to APP and BACE1 mRNAS and the BACE1AS lncRNA and enhance their stabilization (PubMed:24857657). Plays a role in neurite outgrowth and in the establishment and maturation of dendritic arbors, thereby contributing to neocortical and hippocampal circuitry function (By similarity). Stabilizes GAP43 mRNA and protects it from decay during postembryonic development in the brain (PubMed:12034726). By promoting the stabilization of GAP43 mRNA, plays a role in NGF-mediated neurite outgrowth (By similarity). Binds to BDNF long 3'UTR mRNA, thereby leading to its stabilization and increased dendritic translation after activation of PKC (By similarity). By increasing translation of BDNF after nerve injury, may contribute to nerve regeneration (By similarity). Acts as a stabilizing factor by binding to the 3'UTR of NOVA1 mRNA, thereby increasing its translation and enhancing its functional activity in neuron-specific splicing (PubMed:18218628). Stimulates translation of mRNA in a poly(A)- and cap-dependent manner, possibly by associating with the EIF4F cap-binding complex (By similarity). May also negatively regulate translation by binding to the 5'UTR of Ins2 mRNA, thereby repressing its translation (By similarity). Upon glucose stimulation, Ins2 mRNA is released from ELAVL4 and translational inhibition is abolished (By similarity). Also plays a role in the regulation of alternative splicing (PubMed:17035636). May regulate alternative splicing of CALCA pre-mRNA into Calcitonin and Calcitonin gene-related peptide 1 (CGRP) by competing with splicing regulator TIAR for binding to U-rich intronic sequences of CALCA pre-mRNA (PubMed:17035636).
Belongs to the RRM elav family.
Methylated by CARM1, which leads to reduced RNA-binding activity and enhanced interaction with SMN (PubMed:21088113). Methylation at Arg-248 by CARM1 weakens protective binding to the 3'UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state (By similarity). Methylation is inhibited by NGF, which facilitates neurite outgrowth (By similarity).
Blue Ice
-20°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
The HuD protein also known as ELAV-like protein 4 is a neuronal RNA-binding protein with a molecular mass of approximately 40 kDa. It belongs to the ELAV family of proteins which is well-studied for its involvement in mRNA processing. HuD expresses predominantly in the nervous system where it is present in neurons and developing neural tissue. The protein localizes to the cytoplasm where it binds to mRNA influencing the stability and translation of target mRNAs.
HuD is essential in regulating neuronal differentiation and function. The protein binds to adenylate-uridylate-rich elements (AREs) within the 3' untranslated regions (UTR) of specific mRNAs. It stabilizes these mRNAs contributing to the post-transcriptional regulation of genes linked to synaptic plasticity and neuronal development. HuD does not typically form part of a larger complex but interacts with other RNA-binding proteins to exert its regulatory effects.
HuD plays a significant role in neuronal development and synaptic plasticity. It involves in pathways like the MAPK signaling pathway which is critical for cell differentiation and the mTOR pathway which modulates protein synthesis. In these pathways HuD interacts with proteins such as CPEB and FMRP which also have functions related to RNA regulation and neuronal growth.
HuD's function impacts neurological conditions such as Alzheimer's disease and certain types of cancer. Changes in the expression or activity of HuD can influence the progression of Alzheimer's disease by affecting mRNA stability of genes associated with neural health. Additionally abnormal HuD expression links to neuroblastoma a cancer showing high HuD levels promoting uncontrolled cell growth. In these diseases HuD interacts with tau protein and MYCN respectively contributing to disease mechanisms.
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