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AB112300

Recombinant Human Huntingtin protein (GST tag N-Terminus)

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Recombinant Human Huntingtin protein (GST tag N-Terminus) is a Human Fragment protein, in the 81 to 190 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

HD, IT15, HTT, Huntingtin, Huntington disease protein, HD protein

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SDS-PAGE - Recombinant Human Huntingtin protein (GST tag N-Terminus) (AB112300)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Huntingtin protein (GST tag N-Terminus) (AB112300)

ab112300 analysed on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P42858

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB109115

Anti-Huntingtin antibody [EPR5526]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-Huntingtin antibody [EPR5526] (AB109115)

  • 5
  • 4
Cell Lines & Lysates

AB265976

Human HTT (Huntingtin) knockout HeLa cell line

Advanced Validation

Immunocytochemistry/ Immunofluorescence - Human HTT (Huntingtin) knockout HeLa cell line (AB265976)

  • 0
  • 0
Cell Lines & Lysates

AB256946

Human HTT (Huntingtin) knockout HeLa cell lysate

Western blot - Human HTT (Huntingtin) knockout HeLa cell lysate (AB256946)

  • 0
  • 0
Assay Kits

AB223865

Human IL-33 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-33 ELISA Kit (AB223865)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Useful for Antibody Production and Protein Array
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Sequence info

[{"sequence":"AVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALW","proteinLength":"Fragment","predictedMolecularWeight":"37.73 kDa","actualMolecularWeight":null,"aminoAcidEnd":190,"aminoAcidStart":81,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P42858","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Huntingtin. May play a role in microtubule-mediated transport or vesicle function.. Huntingtin, myristoylated N-terminal fragment. Promotes the formation of autophagic vesicles.

Sequence similarities

Belongs to the huntingtin family.

Post-translational modifications

Huntingtin. Cleaved by caspases downstream of the polyglutamine stretch (PubMed:10770929, PubMed:29802276, PubMed:8696339, PubMed:9535906). The resulting N-terminal fragments are cytotoxic and provokes apoptosis (PubMed:10770929).. Huntingtin. Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.. Huntingtin. Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity.. Huntingtin, myristoylated N-terminal fragment. Myristoylated at Gly-551, following proteolytic cleavage at Asp-550.

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Product protocols

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Target data

Huntingtin. May play a role in microtubule-mediated transport or vesicle function.. Huntingtin, myristoylated N-terminal fragment. Promotes the formation of autophagic vesicles.
See full target information HTT
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Product promise

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