AB323063
Recombinant Human ICA 512/PTPRN Protein Standard (His tag)
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Recombinant Human ICA 512/PTPRN Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.
2 Images
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human ICA 512/PTPRN Protein Standard (His tag) (AB323063)
SDS-PAGE analysis of ab323063 under reducing conditions for 2ug protein.
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AB307350
Recombinant Human Plasma Kallikrein 1B Protein (Active)
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Primary Antibodies
AB207750
Anti-ICA 512/PTPRN antibody [EPR20718]
RabMAb|Recombinant|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (AB207750)](https://content.abcam.com/products/images/ica-512-ptprn-antibody-epr20718-ab207750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118248.jpg)
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Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
Sequence info
[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"53.61 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q16849","tags":[{"tag":"His","terminus":"C-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
Properties and storage information
Form
Liquid
Purification technique
Affinity purification His Tag
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
Target data
Plays a role in vesicle-mediated secretory processes (PubMed : 24843546). Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets (By similarity). Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation (PubMed : 24843546). Plays a role in insulin secretion in response to glucose stimuli (PubMed : 24843546). Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain (By similarity). In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (By similarity). Required to maintain normal levels of renin expression and renin release (By similarity). Seems to lack intrinsic enzyme activity (By similarity). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (By similarity).. ICA512-transmembrane fragment. ICA512-TMF regulates dynamics and exocytosis of insulin secretory granules (SGs); binding of ICA512-TMF to SNTB2/beta-2-syntrophin is proposed to restrain SGs mobility and exocytosis by tethering them to the actin cytoskeleton depending on UTRN; the function is inhibited by cytoplasmic ICA512-CFF dimerizing with ICA512-TMF and displacing SNTB2.. ICA512-cleaved cytosolic fragment. ICA512-CCF translocated to the nucleus promotes expression of insulin and other granule-related genes; the function implicates binding to and regulating activity of STAT5B probably by preventing its dephosphorylation and potentially by inducing its sumoylation by recruiting PIAS4 (PubMed : 15596545, PubMed : 16622421, PubMed : 18178618). Enhances pancreatic beta-cell proliferation by converging with signaling by STAT5B and STAT3 (PubMed : 15596545, PubMed : 16622421, PubMed : 18178618). ICA512-CCF located in the cytoplasm regulates dynamics and exocytosis of insulin secretory granules (SGs) by dimerizing with ICA512-TMF and displacing SNTB2 thus enhancing SGs mobility and exocytosis (PubMed : 18824546, PubMed : 20886068).
Alternative Names
ICA3, ICA512, PTPRN, Receptor-type tyrosine-protein phosphatase-like N, R-PTP-N, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA-2, ICA 512
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