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AB276251

Recombinant Human ICAM3 protein (Fc Chimera His Tag)

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(1 Publication)

Recombinant Human ICAM3 protein (Fc Chimera His Tag) is a Human Fragment protein, in the 1 to 485 aa range, expressed in HEK 293 cells, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

CD50, Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, ICAM3

1 Images
SDS-PAGE - Recombinant Human ICAM3 protein (Fc Chimera His Tag) (AB276251)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ICAM3 protein (Fc Chimera His Tag) (AB276251)

SDS-PAGE analysis of ab276251

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P32942

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MATMVPSVLWPRACWTLLVCCLLTPGVQGQEFLLRVEPQNPVLSAGGSLFVNCSTDCPSSEKIALETSLSKELVASGMGWAAFNLSNVTGNSRILCSVYCNGSQITGSSNITVYRLPERVELAPLPPWQPVGQNFTLRCQVEDGSPRTSLTVVLLRWEEELSRQPAVEEPAEVTATVLASRDDHGAPFSCRTELDMQPQGLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSH","proteinLength":"Fragment","predictedMolecularWeight":"77.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":485,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P32942","tags":[{"tag":"Fc","terminus":"C-Terminus"},{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM-3 also known as CD50 or Intercellular Adhesion Molecule 3 is a cell surface glycoprotein. It weighs approximately 120 kilodaltons. ICAM-3 is expressed majorly on leukocytes including lymphocytes monocytes and granulocytes. It is a member of the immunoglobulin superfamily that participates in cell-cell interaction particularly in immune response. By engaging with integrin receptors mainly LFA-1 ICAM-3 plays a role in leukocyte adhesion and signaling.
Biological function summary

ICAM-3 contributes to the immune system’s functionality by mediating intercellular adhesion and cellular signaling. It acts as a ligand for the integrin LFA-1 found on leukocytes which facilitates the initial immune response. ICAM-3 is not a part of a larger complex but works closely with LFA-1 in homing and recruitment of immune cells to inflammation sites. Its interaction with LFA-1 also supports the formation of the immunological synapse an important step in T-cell activation and immune signaling.

Pathways

ICAM-3 contributes to both the immune response and leukocyte extravasation pathways. The interaction with LFA-1 places it within the context of immune cell signaling pathways impacting integrin-mediated signaling. Another pathway involving ICAM-3 is the cell adhesion pathway which is essential for maintaining proper immune surveillance and response. It collaborates with proteins like ICAM-1 in these pathways to ensure effective immune activity.

ICAM-3 is associated with conditions such as inflammatory diseases and certain autoimmune disorders. Its expression levels and binding interactions can affect the severity and progression of such diseases. In autoimmune diseases ICAM-3 interaction with LFA-1 might contribute to the aberrant immune activation observed. Additionally studies have highlighted its role in infections where viruses exploit ICAM proteins to enter or spread showing its importance in pathogenesis-related conditions.
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Specifications

Form

Lyophilized

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General info

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed : 1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590).

Sequence similarities

Belongs to the immunoglobulin superfamily. ICAM family.

Post-translational modifications

Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

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Product protocols

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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed : 1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590).
See full target information ICAM3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific data 11:868 PubMed39127790

2024

An image-based screen for secreted proteins involved in breast cancer G0 cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

William A Weston,Jordan A Holt,Anna J Wiecek,James Pilling,Lovisa Holmberg Schiavone,David M Smith,Maria Secrier,Alexis R Barr
View all publications
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