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AB127252

Recombinant Human ICAM4 protein (His-DHFR tag N-Terminus)

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Recombinant Human ICAM4 protein (His-DHFR tag N-Terminus) is a Human Fragment protein, in the 43 to 210 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CD242, LW, ICAM4, Intercellular adhesion molecule 4, ICAM-4, Landsteiner-Wiener blood group glycoprotein, LW blood group protein

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q14773

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"18.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":210,"aminoAcidStart":43,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q14773","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM4 also known as CD242 functions as an intercellular adhesion molecule with a mass of approximately 42 kDa. It is mainly expressed on erythrocytes and plays a role in cell-cell adhesion. ICAM4 binds selectively with integrins especially those located on the surface of leukocytes and endothelial cells. This protein facilitates critical interactions that allow cells to communicate and engage with their environment efficiently.
Biological function summary

The protein enables erythrocytes to adhere to vascular endothelium by interacting with other molecules such as integrins. It acts independently and not as part of a larger protein complex. ICAM4 serves as an inherent mediator in maintaining the stability and flexibility of red blood cells during circulation ensuring these cells can navigate through capillaries without significant issues.

Pathways

The process of erythrocyte adhesion involves ICAM4's interaction in the immune response system. Particularly it takes part in pathways connected to cell adhesion and signaling that influence leukocyte-endothelial interaction. Proteins like LFA-1 and Mac-1 specific integrins associate with ICAM4 playing essential parts in immunological functions that determine cell adhesion dynamics.

ICAM4 holds an association with sickle cell disease a genetic disorder affecting hemoglobin in erythrocytes. Abnormal adhesion properties linked to ICAM4 exacerbate vascular occlusion contributing to the pathology of the condition. ICAM4 may also relate to malaria as the altered binding interactions impact the sequestration of parasitized erythrocytes. In these disease settings interactions with proteins like integrins contribute significantly to their pathological manifestations.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Functions as a receptor ligand for the monocyte/macrophage-specific ITGAX : ITGB2 integrin complex and mediates erythrophagocytosis (PubMed : 16985175). Receptor ligand of the platelet-specific ITGA2B : ITGB3 integrin complex involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717, PubMed : 15355350). In vitro, is also a receptor ligand of multiple integrin receptors complexes such ITGAL : ITGB2, ITGAM : ITGB2, ITGA5 : ITGB1, ITGA5 : ITGB3 and ITGA5 : ITGB5 integrin complexes (PubMed : 10846180, PubMed : 11435317, PubMed : 15355350).

Sequence similarities

Belongs to the immunoglobulin superfamily. ICAM family.

Post-translational modifications

N- and O-glycosylated.

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Product protocols

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Target data

Functions as a receptor ligand for the monocyte/macrophage-specific ITGAX : ITGB2 integrin complex and mediates erythrophagocytosis (PubMed : 16985175). Receptor ligand of the platelet-specific ITGA2B : ITGB3 integrin complex involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717, PubMed : 15355350). In vitro, is also a receptor ligand of multiple integrin receptors complexes such ITGAL : ITGB2, ITGAM : ITGB2, ITGA5 : ITGB1, ITGA5 : ITGB3 and ITGA5 : ITGB5 integrin complexes (PubMed : 10846180, PubMed : 11435317, PubMed : 15355350).
See full target information ICAM4
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Product promise

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