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Recombinant human ICOS protein (Fc Chimera Active) is a Human Fragment protein, in the 21 to 139 aa range, expressed in CHO, with >=98% purity, < 0.06 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

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Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 0.06 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

E I N G S A N Y E M F I F H N G G V Q I L C K Y P D I V Q Q F K M Q L L K G G Q I L C D L T K T K G S G N T V S I K S L K F C H S Q L S N N S V S F F L Y N L D H S H A N Y Y F C N L S I F D P P P F K V T L T G G Y L H I Y E S Q L C C Q L

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed:9930702). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed:33033255). Acts also as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed:27135603). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (By similarity). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed:30523347). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).

Alternative names

Recommended products

Recombinant human ICOS protein (Fc Chimera Active) is a Human Fragment protein, in the 21 to 139 aa range, expressed in CHO, with >=98% purity, < 0.06 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 0.06 EU/µg

Expression system

CHO cells

Applications

SDS-PAGE, FuncS

Biological activity

Measured by its ability to inhibit human T cell proliferation induced by human CD275:Fc in the presence of anti-CD3.

Accession
Q9Y6W8-1
Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute vial with 100 µL sterile water. Working aliquots are stable for up to 3 months when stored at -20°C.

Concentration
Loading...
Storage buffer

Constituents: PBS

Sequence info

Amino acid sequence

E I N G S A N Y E M F I F H N G G V Q I L C K Y P D I V Q Q F K M Q L L K G G Q I L C D L T K T K G S G N T V S I K S L K F C H S Q L S N N S V S F F L Y N L D H S H A N Y Y F C N L S I F D P P P F K V T L T G G Y L H I Y E S Q L C C Q L

Accession

Q9Y6W8

Protein length

Fragment

Amino acids

21 to 139

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed:9930702). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed:33033255). Acts also as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed:27135603). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (By similarity). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed:30523347). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).

Post-translational modifications

N-glycosylated.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Inducible T-cell costimulator (ICOS) also known as AILIM or CD278 is a member of the CD28 superfamily of proteins. ICOS is a surface receptor protein approximately 60 kDa in mass. This protein is expressed mainly on activated T cells. It does not appear on naive or resting T cells. ICOS plays an important mechanical role in the immune response as its engagement enhances the production of cytokines and supports cell-cell interactions through its binding to the ICOS ligand.

Biological function summary

ICOS plays a critical role in the regulation of immune responses. It acts as a costimulatory signal for T cells supporting their proliferation and differentiation. ICOS helps form part of a complex involving interactions with the ICOS ligand present on antigen-presenting cells. This interaction affects the function and fate of various T cell subsets including T follicular helper cells which are important for antibody production.

Pathways

ICOS is part of the immune signaling pathway. It associates closely with the CD28 signaling pathway enhancing T-cell activation and survival. ICOS interacts with proteins such as the ICOS ligand (ICOSL) on antigen-presenting cells which helps mediate its effects. These pathways are essential in immune system homeostasis and adaptive immune responses where dysfunction can lead to immune-related diseases.

Associated diseases and disorders

ICOS has implications in conditions such as autoimmune diseases and cancer. Abnormal expression or function of ICOS relates to the development of autoimmune conditions like systemic lupus erythematosus (SLE) where the overactive immune response damages the body's tissues. Additionally ICOS might support tumor progression in certain types of cancers by promoting immune escape. It interacts with regulatory proteins like CTLA-4 and PD-1 in these diseases which are notably involved in immune checkpoint pathways.

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