Recombinant human IDH1 (mutated R132H) protein is a Human Full Length protein, in the 1 to 414 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M S K K I S G G S V V E M Q G D E M T R I I W E L I K E K L I F P Y V E L D L H S Y D L G I E N R D A T N D Q V T K D A A E A I K K H N V G V K C A T I T P D E K R V E E F K L K Q M W K S P N G T I R N I L G G T V F R E A I I C K N I P R L V S G W V K P I I I G H H A Y G D Q Y R A T D F V V P G P G K V E I T Y T P S D G T Q K V T Y L V H N F E E G G G V A M G M Y N Q D K S I E D F A H S S F Q M A L S K G W P L Y L S T K N T I L K K Y D G R F K D I F Q E I Y D K Q Y K S Q F E A Q K I W Y E H R L I D D M V A Q A M K S E G G F I W A C K N Y D G D V Q S D S V A Q G Y G S L G M M T S V L V C P D G K T V E A E A A H G T V T R H Y R M Y Q K G Q E T S T N P I A S I F A W T R G L A H R A K L D N N K E L A F F A N A L E E V S I E T I E A G F M T K D L A A C I K G L P N V Q R S D Y L N T F E F M D K L G E N L K I K L A Q A K L D Y K D D D D K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Alternative names
PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
Recommended products
Recombinant human IDH1 (mutated R132H) protein is a Human Full Length protein, in the 1 to 414 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 53 pmol/min/μg.
Assay conditions: ab198123 reductive activity was measured in 200 μl reaction containing 25 mM Tris (pH 7.4), 150 mM NaCl, 10 mM MgCl2, 0.03% BSA, 1 mM -Ketoglutarate, 10 μM NADPH and IDH. Depletion of NADPH was monitored over time at 340 nm.
Depletion of NADPH was monitored continuously at Abs340 nm for 20 min. Molar extinction coefficient of NADPH is 6,200
M-1cm-1. - Accession
- O75874-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M S K K I S G G S V V E M Q G D E M T R I I W E L I K E K L I F P Y V E L D L H S Y D L G I E N R D A T N D Q V T K D A A E A I K K H N V G V K C A T I T P D E K R V E E F K L K Q M W K S P N G T I R N I L G G T V F R E A I I C K N I P R L V S G W V K P I I I G H H A Y G D Q Y R A T D F V V P G P G K V E I T Y T P S D G T Q K V T Y L V H N F E E G G G V A M G M Y N Q D K S I E D F A H S S F Q M A L S K G W P L Y L S T K N T I L K K Y D G R F K D I F Q E I Y D K Q Y K S Q F E A Q K I W Y E H R L I D D M V A Q A M K S E G G F I W A C K N Y D G D V Q S D S V A Q G Y G S L G M M T S V L V C P D G K T V E A E A A H G T V T R H Y R M Y Q K G Q E T S T N P I A S I F A W T R G L A H R A K L D N N K E L A F F A N A L E E V S I E T I E A G F M T K D L A A C I K G L P N V Q R S D Y L N T F E F M D K L G E N L K I K L A Q A K L D Y K D D D D K
- Accession
- O75874
- Protein length
- Full Length
- Predicted molecular weight
- 48 kDa
- Amino acids
- 1 to 414
- Nature
- Recombinant
- Tags
- DDDDK tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
- Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
- Post-translational modifications
Acetylation at Lys-374 dramatically reduces catalytic activity.
- Subcellular localisation
- Peroxisome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as Isocitrate dehydrogenase
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.
- Biological function summary
Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.
- Pathways
The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.
- Associated diseases and disorders
IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.
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2 product images
Functional Studies - Recombinant human IDH1 (mutated R132H) protein (ab198123)
Specific activity of ab198123 was determined to be 53 pmol/min/μg
SDS-PAGE - Recombinant human IDH1 (mutated R132H) protein (ab198123)
4-20% SDS-PAGE analysis of 1.6 μg ab198123 with Coomassie staining.
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