Skip to main content

Recombinant human IDH1 protein is a Human Full Length protein, in the 1 to 414 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant human IDH1 protein (AB113858), expandable thumbnail

Publications

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

Yes

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M S K K I S G G S V V E M Q G D E M T R I I W E L I K E K L I F P Y V E L D L H S Y D L G I E N R D A T N D Q V T K D A A E A I K K H N V G V K C A T I T P D E K R V E E F K L K Q M W K S P N G T I R N I L G G T V F R E A I I C K N I P R L V S G W V K P I I I G R H A Y G D Q Y R A T D F V V P G P G K V E I T Y T P S D G T Q K V T Y L V H N F E E G G G V A M G M Y N Q D K S I E D F A H S S F Q M A L S K G W P L Y L S T K N T I L K K Y D G R F K D I F Q E I Y D K Q Y K S Q F E A Q K I W Y E H R L I D D M V A Q A M K S E G G F I W A C K N Y D G D V Q S D S V A Q G Y G S L G M M T S V L V C P D G K T V E A E A A H G T V T R H Y R M Y Q K G Q E T S T N P I A S I F A W T R G L A H R A K L D N N K E L A F F A N A L E E V S I E T I E A G F M T K D L A A C I K G L P N V Q R S D Y L N T F E F M D K L G E N L K I K L A Q A K L

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

Alternative names

Recommended products

Recombinant human IDH1 protein is a Human Full Length protein, in the 1 to 414 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Biological activity

Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C. Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.

Accession
O75874-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M S K K I S G G S V V E M Q G D E M T R I I W E L I K E K L I F P Y V E L D L H S Y D L G I E N R D A T N D Q V T K D A A E A I K K H N V G V K C A T I T P D E K R V E E F K L K Q M W K S P N G T I R N I L G G T V F R E A I I C K N I P R L V S G W V K P I I I G R H A Y G D Q Y R A T D F V V P G P G K V E I T Y T P S D G T Q K V T Y L V H N F E E G G G V A M G M Y N Q D K S I E D F A H S S F Q M A L S K G W P L Y L S T K N T I L K K Y D G R F K D I F Q E I Y D K Q Y K S Q F E A Q K I W Y E H R L I D D M V A Q A M K S E G G F I W A C K N Y D G D V Q S D S V A Q G Y G S L G M M T S V L V C P D G K T V E A E A A H G T V T R H Y R M Y Q K G Q E T S T N P I A S I F A W T R G L A H R A K L D N N K E L A F F A N A L E E V S I E T I E A G F M T K D L A A C I K G L P N V Q R S D Y L N T F E F M D K L G E N L K I K L A Q A K L

Accession

O75874

Protein length

Full Length

Predicted molecular weight

48.8 kDa

Amino acids

1 to 414

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab113858 was purified using conventional chromatography.

General info

Function

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Post-translational modifications

Acetylation at Lys-374 dramatically reduces catalytic activity.

Subcellular localisation

Peroxisome

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This product was previously labelled as Isocitrate dehydrogenase

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.

Biological function summary

Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

Pathways

The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

Associated diseases and disorders

IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant human IDH1 protein (ab113858), expandable thumbnail

    SDS-PAGE - Recombinant human IDH1 protein (ab113858)

    3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com