Recombinant human IDH1 protein is a Human Full Length protein, in the 1 to 414 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
Yes
M G S S H H H H H H S S G L V P R G S H M S K K I S G G S V V E M Q G D E M T R I I W E L I K E K L I F P Y V E L D L H S Y D L G I E N R D A T N D Q V T K D A A E A I K K H N V G V K C A T I T P D E K R V E E F K L K Q M W K S P N G T I R N I L G G T V F R E A I I C K N I P R L V S G W V K P I I I G R H A Y G D Q Y R A T D F V V P G P G K V E I T Y T P S D G T Q K V T Y L V H N F E E G G G V A M G M Y N Q D K S I E D F A H S S F Q M A L S K G W P L Y L S T K N T I L K K Y D G R F K D I F Q E I Y D K Q Y K S Q F E A Q K I W Y E H R L I D D M V A Q A M K S E G G F I W A C K N Y D G D V Q S D S V A Q G Y G S L G M M T S V L V C P D G K T V E A E A A H G T V T R H Y R M Y Q K G Q E T S T N P I A S I F A W T R G L A H R A K L D N N K E L A F F A N A L E E V S I E T I E A G F M T K D L A A C I K G L P N V Q R S D Y L N T F E F M D K L G E N L K I K L A Q A K L
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, IDH1, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
Recombinant human IDH1 protein is a Human Full Length protein, in the 1 to 414 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
Yes
Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C. Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.
No
Human
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
M G S S H H H H H H S S G L V P R G S H M S K K I S G G S V V E M Q G D E M T R I I W E L I K E K L I F P Y V E L D L H S Y D L G I E N R D A T N D Q V T K D A A E A I K K H N V G V K C A T I T P D E K R V E E F K L K Q M W K S P N G T I R N I L G G T V F R E A I I C K N I P R L V S G W V K P I I I G R H A Y G D Q Y R A T D F V V P G P G K V E I T Y T P S D G T Q K V T Y L V H N F E E G G G V A M G M Y N Q D K S I E D F A H S S F Q M A L S K G W P L Y L S T K N T I L K K Y D G R F K D I F Q E I Y D K Q Y K S Q F E A Q K I W Y E H R L I D D M V A Q A M K S E G G F I W A C K N Y D G D V Q S D S V A Q G Y G S L G M M T S V L V C P D G K T V E A E A A H G T V T R H Y R M Y Q K G Q E T S T N P I A S I F A W T R G L A H R A K L D N N K E L A F F A N A L E E V S I E T I E A G F M T K D L A A C I K G L P N V Q R S D Y L N T F E F M D K L G E N L K I K L A Q A K L
Full Length
48.8 kDa
1 to 414
Recombinant
His tag N-Terminus
Liquid
ab113858 was purified using conventional chromatography.
Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Acetylation at Lys-374 dramatically reduces catalytic activity.
Peroxisome
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This product was previously labelled as Isocitrate dehydrogenase
This supplementary information is collated from multiple sources and compiled automatically.
IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.
Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.
The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.
IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com