Recombinant human IDH2 protein is a Human Full Length protein, in the 1 to 452 aa range, expressed in Baculovirus infected Sf9, with >=59% purity and suitable for SDS-PAGE, FuncS.
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- Nature 559:125-1292018Acquired resistance to IDH inhibition through trans or cis dimer-interface mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew M Intlekofer,Alan H Shih,Bo Wang,Abbas Nazir,Ariën S Rustenburg,Steven K Albanese,Minal Patel,Christopher Famulare,Fabian M Correa,Naofumi Takemoto,Vidushi Durani,Hui Liu,Justin Taylor,Noushin Farnoud,Elli Papaemmanuil,Justin R Cross,Martin S Tallman,Maria E Arcila,Mikhail Roshal,Gregory A Petsko,Bin Wu,Sung Choe,Zenon D Konteatis,Scott A Biller,John D Chodera,Craig B Thompson,Ross L Levine,Eytan M SteinPubMed 29950729
Key facts
- Purity
- >=59% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A G Y L R V V R S L C R A S G S R P A W A P A A L T A P T S Q E Q P R R H Y A D K R I K V A K P V V E M D G D E M T R I I W Q F I K E K L I L P H V D I Q L K Y F D L G L P N R D Q T D D Q V T I D S A L A T Q K Y S V A V K C A T I T P D E A R V E E F K L K K M W K S P N G T I R N I L G G T V F R E P I I C K N I P R L V P G W T K P I T I G R H A H G D Q Y K A T D F V A D R A G T F K M V F T P K D G S G V K E W E V Y N F P A G G V G M G M Y N T D E S I S G F A H S C F Q Y A I Q K K W P L Y M S T K N T I L K A Y D G R F K D I F Q E I F D K H Y K T D F D K N K I W Y E H R L I D D M V A Q V L K S S G G F V W A C K N Y D G D V Q S D I L A Q G F G S L G L M T S V L V C P D G K T I E A E A A H G T V T R H Y R E H Q K G R P T S T N P I A S I F A W T R G L E H R G K L D G N Q D L I R F A Q M L E K V C V E T V E S G A M T K D L A G C I H G L S N V K L N E H F L N T T D F L D T I K S N L D R A L G R Q D Y K D D D D K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a role in intermediary metabolism and energy production (PubMed:19228619, PubMed:22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed:19228619, PubMed:22416140).
Alternative names
IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2
Recommended products
Recombinant human IDH2 protein is a Human Full Length protein, in the 1 to 452 aa range, expressed in Baculovirus infected Sf9, with >=59% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=59% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 14,200 pmol/min/μg.
Assay Conditions:
The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl2, 200 μM DL-isocitrate, 200 μM NADP+, and various amount of ab198092 in a volume of 200 μL. Reactions were initiated with the addition of ab198092, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M-1cm-1.
. - Accession
- P48735-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M A G Y L R V V R S L C R A S G S R P A W A P A A L T A P T S Q E Q P R R H Y A D K R I K V A K P V V E M D G D E M T R I I W Q F I K E K L I L P H V D I Q L K Y F D L G L P N R D Q T D D Q V T I D S A L A T Q K Y S V A V K C A T I T P D E A R V E E F K L K K M W K S P N G T I R N I L G G T V F R E P I I C K N I P R L V P G W T K P I T I G R H A H G D Q Y K A T D F V A D R A G T F K M V F T P K D G S G V K E W E V Y N F P A G G V G M G M Y N T D E S I S G F A H S C F Q Y A I Q K K W P L Y M S T K N T I L K A Y D G R F K D I F Q E I F D K H Y K T D F D K N K I W Y E H R L I D D M V A Q V L K S S G G F V W A C K N Y D G D V Q S D I L A Q G F G S L G L M T S V L V C P D G K T I E A E A A H G T V T R H Y R E H Q K G R P T S T N P I A S I F A W T R G L E H R G K L D G N Q D L I R F A Q M L E K V C V E T V E S G A M T K D L A G C I H G L S N V K L N E H F L N T T D F L D T I K S N L D R A L G R Q D Y K D D D D K
- Accession
- P48735
- Protein length
- Full Length
- Predicted molecular weight
- 52 kDa
- Amino acids
- 1 to 452
- Nature
- Recombinant
- Tags
- DDDDK tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Plays a role in intermediary metabolism and energy production (PubMed:19228619, PubMed:22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed:19228619, PubMed:22416140).
- Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
- Post-translational modifications
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.
- Biological function summary
The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.
- Pathways
The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.
- Associated diseases and disorders
The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.
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2 product images
Functional Studies - Recombinant human IDH2 protein (ab198092)
Activity assay of ab198092.
SDS-PAGE - Recombinant human IDH2 protein (ab198092)
10% SDS-PAGE analysis of 1.4 μg ab198092 with Coomassie staining.
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