Recombinant Human IDI1 protein is a Human Full Length protein, in the 1 to 228 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M M P E I N T N H L D K Q Q V Q L L A E M C I L I D E N D N K I G A E T K K N C H L N E N I E K G L L H R A F S V F L F N T E N K L L L Q Q R S D A K I T F P G C F T N T C C S H P L S N P A E L E E S D A L G V R R A A Q R R L K A E L G I P L E E V P P E E I N Y L T R I H Y K A Q S D G I W G E H E I D Y I L L V R K N V T L N P D P N E I K S Y C Y V S K E E L K E L L K K A A S G E I K I T P W F K I I A A T F L F K W W D N L N H L N Q F V D H E K I Y R M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).
Alternative names
Isopentenyl-diphosphate Delta-isomerase 1, Isopentenyl pyrophosphate isomerase 1, IPP isomerase 1, IPPI1, IDI1
Recommended products
Recombinant Human IDI1 protein is a Human Full Length protein, in the 1 to 228 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q13907-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M M P E I N T N H L D K Q Q V Q L L A E M C I L I D E N D N K I G A E T K K N C H L N E N I E K G L L H R A F S V F L F N T E N K L L L Q Q R S D A K I T F P G C F T N T C C S H P L S N P A E L E E S D A L G V R R A A Q R R L K A E L G I P L E E V P P E E I N Y L T R I H Y K A Q S D G I W G E H E I D Y I L L V R K N V T L N P D P N E I K S Y C Y V S K E E L K E L L K K A A S G E I K I T P W F K I I A A T F L F K W W D N L N H L N Q F V D H E K I Y R M
- Accession
- Q13907
- Protein length
- Full Length
- Predicted molecular weight
- 28.6 kDa
- Amino acids
- 1 to 228
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113598 was purified using conventional chromatography.
General info
- Function
Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).
- Sequence similarities
Belongs to the IPP isomerase type 1 family.
- Subcellular localisation
- Peroxisome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IDI1 also known as Isopentenyl-diphosphate delta isomerase 1 plays an important role in isoprenoid biosynthesis. The protein facilitates the conversion of isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP) an essential step in the biosynthetic pathway. This enzyme has a molecular mass of approximately 44 kDa. You can find IDI1 expressions mainly in the liver though it is also noted in other tissues including the heart and kidneys.
- Biological function summary
IDI1 contributes to the formation of isoprenoids which are essential for numerous cellular functions like cholesterol synthesis and protein prenylation. It principally operates as a monomer and does not typically associate with a larger protein complex during its activity. The enzyme's role in positioning alongside other essential enzymes makes it significant for the transcriptional regulation of certain metabolic processes.
- Pathways
IDI1 participates extensively in the mevalonate pathway a fundamental route for cholesterol biosynthesis. It functions under the regulation of proteins like HMG-CoA reductase a rate-limiting enzyme in this pathway. The enzyme also connects to the isoprenoid biosynthesis pathway where it interacts with proteins involved in the synthesis of farnesyl pyrophosphate a precursor in the generation of various biomolecules.
- Associated diseases and disorders
IDI1 links with metabolic conditions such as hypercholesterolemia and Smith-Lemli-Opitz syndrome both affecting cholesterol levels. The interaction with proteins such as DHCR7 involved in the conversion of 7-dehydrocholesterol to cholesterol highlights its implication in these disorders. Dysfunction in IDI1 activity might lead to the disruption of regular metabolic balance further impacting cellular health.
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1 product image
SDS-PAGE - Recombinant Human IDI1 protein (ab113598)
15% SDS-PAGE analysis of ab113598 (3μg)
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