JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB216243

Recombinant human IDO-2 protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human IDO-2 protein (His tag) is a Human Fragment protein, in the 15 to 420 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

INDOL1, IDO2, IDO-2

2 Images
Functional Studies - Recombinant human IDO-2 protein (His tag) (AB216243)
  • FuncS

Supplier Data

Functional Studies - Recombinant human IDO-2 protein (His tag) (AB216243)

Activity assay for ab216243.

SDS-PAGE - Recombinant human IDO-2 protein (His tag) (AB216243)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IDO-2 protein (His tag) (AB216243)

4-20% SDS-PAGE analysis of ab216243 (4.2 μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

IDO-2 activity was confirmed using an IDO2 inhibitor screening assay Kit

Accession

Q6ZQW0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.02% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Protein previously labeled as INDOL1.
style
left-column

Sequence info

[{"sequence":"EPHRPNVKTAVPLSLESYHISEEYGFLLPDSLKELPDHYRPWMEIANKLPQLIDAHQLQAHVDKMPLLSCQFLKGHREQRLAHLVLSFLTMGYVWQEGEAQPAEVLPRNLALPFVEVSRNLGLPPILVHSDLVLTNWTKKDPDGFLEIGNLETIISFPGGESLHGFILVTALVEKEAVPGIKALVQATNAILQPNQEALLQALQRLRLSIQDITKTLGQMHDYVDPDIFYAGIRIFLSGWKDNPAMPAGLMYEGVSQEPLKYSGGSAAQSTVLHAFDEFLGIRHSKESGDFLYRMRDYMPPSHKAFIEDIHSAPSLRDYILSSGQDHLLTAYNQCVQALAELRSYHITMVTKYLITAAAKAKHGKPNHLPGPPQALKDRGTGGTAVMSFLKSVRDKTLESILHPRG","proteinLength":"Fragment","predictedMolecularWeight":"46 kDa","actualMolecularWeight":null,"aminoAcidEnd":420,"aminoAcidStart":15,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6ZQW0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 23-dioxygenase 2 (IDO-2) is an enzyme involved in tryptophan catabolism. IDO-2 is also known as INDOL1 or INDOLE-2. This enzyme differs from its counterpart IDO1 in its substrate specificity and tissue distribution. IDO-2 has a molecular mass of approximately 45 kDa. It is expressed in various tissues including liver kidney and placenta with notably higher expression in immune system cells like dendritic cells and macrophages.
Biological function summary

IDO-2 participates in the degradation of the essential amino acid tryptophan via the kynurenine pathway. This process helps modulate immune responses by reducing tryptophan availability and producing metabolites that affect T-cell function. IDO-2 is not known to be part of a larger protein complex but it does work in parallel with IDO1 to contribute to immune tolerance and homeostasis within the body.

Pathways

The kynurenine pathway prominently involves IDO-2 where it aids in the conversion of tryptophan to kynurenine influencing pathways related to immune regulation. IDO-2 interacts closely with enzymes such as tryptophan 23-dioxygenase (TDO) sharing roles in tryptophan metabolism. Both IDO2 and IDO1 can lead to the production of kynurenine highlighting their shared influence within this metabolic pathway.

The activity of IDO-2 plays a role in conditions like cancer and autoimmune diseases. In cancer its ability to suppress immune attack on tumors by depleting tryptophan and generating immunosuppressive metabolites allows tumor cells to escape immune surveillance. In autoimmune disorders like systemic lupus erythematosus altered tryptophan metabolism through IDO-2 involvement can affect disease progression. IDO-2 works alongside IDO1 within these pathological contexts influencing disease outcomes due to their shared enzymatic roles in immune modulation.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in immune regulation. May not play a significant role in tryptophan-related tumoral resistance (PubMed : 25691885).

Sequence similarities

Belongs to the indoleamine 2,3-dioxygenase family.

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in immune regulation. May not play a significant role in tryptophan-related tumoral resistance (PubMed : 25691885).
See full target information IDO2
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB235936

Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit

Functional Studies - Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit (AB235936)

  • 0
  • 0
Primary Antibodies

AB311847

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR28349-89]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR28349-89] (AB311847)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Assay Kits

AB235936

Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit

Functional Studies - Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit (AB235936)

  • 0
  • 0
Assay Kits

AB245710

Human IDO ELISA Kit

Recombinant|SimpleStep

ELISA - Human IDO ELISA Kit (AB245710)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com