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AB313356

Recombinant Human IDO1 Protein (His tag) (E.Coli)

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Recombinant Human IDO1 Protein (His tag) (E.Coli) is a Human Full Length protein, in the 2 to 403 aa range, expressed in Escherichia coli, with >95%, <0.1 EU/mg endotoxin level, suitable for HPLC, SDS-PAGE, Mass Spec.

View Alternative Names

IDO, INDO, IDO1, IDO-1

3 Images
Mass Spectrometry - Recombinant Human IDO1 Protein (His tag) (E.Coli) (AB313356)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Human IDO1 Protein (His tag) (E.Coli) (AB313356)

Mass determination by ESI-TOF. Predicted MW is 46149.57 (+/- 10Da by ESI-TOF). Observed MW is 46149.58.

HPLC - Recombinant Human IDO1 Protein (His tag) (E.Coli) (AB313356)
  • HPLC

Lab

HPLC - Recombinant Human IDO1 Protein (His tag) (E.Coli) (AB313356)

HPLC analysis of ab313356

SDS-PAGE - Recombinant Human IDO1 Protein (His tag) (E.Coli) (AB313356)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IDO1 Protein (His tag) (E.Coli) (AB313356)

SDS-page analysis of ab313356

Key facts

Purity

>95% HPLC

Endotoxin level

<0.1 EU/mg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec, HPLC

applications

Biologically active

No

Accession

P14902

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.7293% Sodium chloride, 0.5769% Tris

storage-buffer

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Complementary Products

Primary Antibodies

AB211017

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (AB211017)

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Proteins & Peptides

AB128443

Recombinant Human ULBP2 protein (denatured)

SDS-PAGE - Recombinant Human ULBP2 protein (denatured) (AB128443)

  • 0
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Proteins & Peptides

AB134517

Recombinant Human ULBP1 protein

SDS-PAGE - Recombinant Human ULBP1 protein (AB134517)

  • 0
  • 0
Assay Kits

AB235936

Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit

Functional Studies - Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit (AB235936)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG","proteinLength":"Full Length","predictedMolecularWeight":"46.1 kDa","actualMolecularWeight":"46.1 kDa","aminoAcidEnd":403,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P14902","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.
Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174, PubMed : 18026683). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).

Sequence similarities

Belongs to the indoleamine 2,3-dioxygenase family.

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Product protocols

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Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174, PubMed : 18026683). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).
See full target information IDO1
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