Recombinant Human IDOL protein is a Human Full Length protein, in the 2 to 445 aa range, expressed in Baculovirus infected Sf9, with >=89% purity and suitable for SDS-PAGE.
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- Endocrine connections 11:2022Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelody Lok-Yi Chan,Sammy Wing-Ming Shiu,Ching-Lung Cheung,Anskar Yu-Hung Leung,Kathryn Choon-Beng TanPubMed 35560019
Key facts
- Purity
- >=89% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His-DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M H H H H H H D Y K D D D D K L C Y V T R P D A V L M E V E V E A K A N G E D C L N Q V C R R L G I I E V D Y F G L Q F T G S K G E S L W L N L R N R I S Q Q M D G L A P Y R L K L R V K F F V E P H L I L Q E Q T R H I F F L H I K E A L L A G H L L C S P E Q A V E L S A L L A Q T K F G D Y N Q N T A K Y N Y E E L C A K E L S S A T L N S I V A K H K E L E G T S Q A S A E Y Q V L Q I V S A M E N Y G I E W H S V R D S E G Q K L L I G V G P E G I S I C K D D F S P I N R I A Y P V V Q M A T Q S G K N V Y L T V T K E S G N S I V L L F K M I S T R A A S G L Y R A I T E T H A F Y R C D T V T S A V M M Q Y S R D L K G H L A S L F L N E N I N L G K K Y V F D I K R T S K E V Y D H A R R A L Y N A G V V D L V S R N N Q S P S H S P L K S S E S S M N C S S C E G L S C Q Q T R V L Q E K L R K L K E A M L C M V C C E E E I N S T F C P C G H T V C C E S C A A Q L Q S C P V C R S R V E H V Q H V Y L P T H T S L L N L T V I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
Alternative names
BZF1, IDOL, BM-023, PP5242, MYLIP, E3 ubiquitin-protein ligase MYLIP, Inducible degrader of the LDL-receptor, Myosin regulatory light chain interacting protein, RING-type E3 ubiquitin transferase MYLIP, Idol, MIR
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Recombinant Human IDOL protein is a Human Full Length protein, in the 2 to 445 aa range, expressed in Baculovirus infected Sf9, with >=89% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=89% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His-DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8WY64-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.017% Potassium chloride
Sequence info
Amino acid sequence
- M H H H H H H D Y K D D D D K L C Y V T R P D A V L M E V E V E A K A N G E D C L N Q V C R R L G I I E V D Y F G L Q F T G S K G E S L W L N L R N R I S Q Q M D G L A P Y R L K L R V K F F V E P H L I L Q E Q T R H I F F L H I K E A L L A G H L L C S P E Q A V E L S A L L A Q T K F G D Y N Q N T A K Y N Y E E L C A K E L S S A T L N S I V A K H K E L E G T S Q A S A E Y Q V L Q I V S A M E N Y G I E W H S V R D S E G Q K L L I G V G P E G I S I C K D D F S P I N R I A Y P V V Q M A T Q S G K N V Y L T V T K E S G N S I V L L F K M I S T R A A S G L Y R A I T E T H A F Y R C D T V T S A V M M Q Y S R D L K G H L A S L F L N E N I N L G K K Y V F D I K R T S K E V Y D H A R R A L Y N A G V V D L V S R N N Q S P S H S P L K S S E S S M N C S S C E G L S C Q Q T R V L Q E K L R K L K E A M L C M V C C E E E I N S T F C P C G H T V C C E S C A A Q L Q S C P V C R S R V E H V Q H V Y L P T H T S L L N L T V I
- Accession
- Q8WY64
- Protein length
- Full Length
- Predicted molecular weight
- 15.7 kDa
- Amino acids
- 2 to 445
- Nature
- Recombinant
- Tags
- His-DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
- Post-translational modifications
Autoubiquitinated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IDOL known as Inducible Degrader of the Low-density lipoprotein receptor plays an important role in protein degradation especially the degradation of the LDL receptor (LDLR). The IDOL protein also referred to as MYLIP (Myosin Regulatory Light Chain Interacting Protein) has a molecular mass of approximately 54 kDa. It is widely expressed in tissues with notable expression in the brain liver and macrophages. IDOL functions as an E3 ubiquitin ligase tagging LDLR for degradation which subsequently influences cholesterol levels in the body.
- Biological function summary
Within cellular systems IDOL regulates cholesterol homeostasis by controlling the degradation of LDL receptors. It is not directly part of a large protein complex but interacts with other proteins involved in lipid metabolism. By mediating LDLR degradation IDOL indirectly affects cholesterol uptake by cells. IDOL's regulatory mechanism is vital in preventing excessive cellular cholesterol accumulation which can lead to various metabolic problems if unregulated.
- Pathways
IDOL serves an important role in the lipid metabolic pathways and is part of the broader cholesterol efflux regulatory network. IDOL acts downstream of the Liver X Receptor (LXR) pathway. The LXR upon activation by oxysterols promotes IDOL expression leading to enhanced LDL receptor degradation. This pathway makes IDOL an important regulator of cholesterol levels linking it with proteins like LDLR and the LXRs. In this regulatory network IDOL ensures balanced cholesterol levels by modulating receptor availability and cholesterol uptake in the liver and peripheral tissues.
- Associated diseases and disorders
IDOL is implicated in conditions such as hypercholesterolemia and atherosclerosis due to its cholesterol regulatory functions. Alterations in IDOL activity or expression can lead to abnormal cholesterol buildup raising the risk of cardiovascular diseases. The connection between IDOL and LDLR is critical as both proteins influence cholesterol metabolism disorders. Mutations or dysregulation affecting IDOL can disturb normal lipid homeostasis consequently contributing to disease progression. Understanding IDOL’s mechanism further opens avenues for potential therapeutic interventions targeting cholesterol-related diseases.
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SDS-PAGE - Recombinant Human IDOL protein (ab198638)
4-20% SDS-PAGE with Coomassie staining
Lane 1: 0.9 μg ab198638
Lane 2: Protein marker
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