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AB198638

Recombinant Human IDOL protein (His-DDDDK tag N-Terminus)

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(1 Publication)

Recombinant Human IDOL protein (His-DDDDK tag N-Terminus) is a Human Full Length protein, in the 2 to 445 aa range, expressed in Baculovirus infected Sf9 cells, with >89%, suitable for SDS-PAGE.

View Alternative Names

BZF1, IDOL, BM-023, PP5242, MYLIP, E3 ubiquitin-protein ligase MYLIP, Inducible degrader of the LDL-receptor, Myosin regulatory light chain interacting protein, RING-type E3 ubiquitin transferase MYLIP, Idol, MIR

1 Images
SDS-PAGE - Recombinant Human IDOL protein (His-DDDDK tag N-Terminus) (AB198638)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IDOL protein (His-DDDDK tag N-Terminus) (AB198638)

4-20% SDS-PAGE with Coomassie staining

Lane 1 : 0.9 μg ab198638
Lane 2 : Protein marker

Key facts

Purity

>89% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His-DDDDK tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8WY64

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.017% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB74562

Anti-IDOL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDOL antibody (AB74562)

  • 4
  • 4
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
Proteins & Peptides

AB318256

Recombinant Clostridioides difficile Toxin B/TcdB Protein (His-tag)

Mass Spectrometry - Recombinant Clostridioides difficile Toxin B/TcdB Protein (His-tag) (AB318256)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MHHHHHHDYKDDDDKLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKEALLAGHLLCSPEQAVELSALLAQTKFGDYNQNTAKYNYEELCAKELSSATLNSIVAKHKELEGTSQASAEYQVLQIVSAMENYGIEWHSVRDSEGQKLLIGVGPEGISICKDDFSPINRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAGVVDLVSRNNQSPSHSPLKSSESSMNCSSCEGLSCQQTRVLQEKLRKLKEAMLCMVCCEEEINSTFCPCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI","proteinLength":"Full Length","predictedMolecularWeight":"15.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":445,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8WY64","tags":[{"tag":"His-DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDOL known as Inducible Degrader of the Low-density lipoprotein receptor plays an important role in protein degradation especially the degradation of the LDL receptor (LDLR). The IDOL protein also referred to as MYLIP (Myosin Regulatory Light Chain Interacting Protein) has a molecular mass of approximately 54 kDa. It is widely expressed in tissues with notable expression in the brain liver and macrophages. IDOL functions as an E3 ubiquitin ligase tagging LDLR for degradation which subsequently influences cholesterol levels in the body.
Biological function summary

Within cellular systems IDOL regulates cholesterol homeostasis by controlling the degradation of LDL receptors. It is not directly part of a large protein complex but interacts with other proteins involved in lipid metabolism. By mediating LDLR degradation IDOL indirectly affects cholesterol uptake by cells. IDOL's regulatory mechanism is vital in preventing excessive cellular cholesterol accumulation which can lead to various metabolic problems if unregulated.

Pathways

IDOL serves an important role in the lipid metabolic pathways and is part of the broader cholesterol efflux regulatory network. IDOL acts downstream of the Liver X Receptor (LXR) pathway. The LXR upon activation by oxysterols promotes IDOL expression leading to enhanced LDL receptor degradation. This pathway makes IDOL an important regulator of cholesterol levels linking it with proteins like LDLR and the LXRs. In this regulatory network IDOL ensures balanced cholesterol levels by modulating receptor availability and cholesterol uptake in the liver and peripheral tissues.

IDOL is implicated in conditions such as hypercholesterolemia and atherosclerosis due to its cholesterol regulatory functions. Alterations in IDOL activity or expression can lead to abnormal cholesterol buildup raising the risk of cardiovascular diseases. The connection between IDOL and LDLR is critical as both proteins influence cholesterol metabolism disorders. Mutations or dysregulation affecting IDOL can disturb normal lipid homeostasis consequently contributing to disease progression. Understanding IDOL’s mechanism further opens avenues for potential therapeutic interventions targeting cholesterol-related diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.

Post-translational modifications

Autoubiquitinated.

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Product protocols

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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
See full target information MYLIP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Endocrine connections 11: PubMed35560019

2022

Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Lok-Yi Chan,Sammy Wing-Ming Shiu,Ching-Lung Cheung,Anskar Yu-Hung Leung,Kathryn Choon-Beng Tan
View all publications
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