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AB158721

Recombinant Human Iduronate 2 sulfatase/SIDS protein (GST tag N-Terminus)

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Recombinant Human Iduronate 2 sulfatase/SIDS protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 312 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

SIDS, IDS, Iduronate 2-sulfatase, Alpha-L-iduronate sulfate sulfatase, Idursulfase

1 Images
SDS-PAGE - Recombinant Human Iduronate 2 sulfatase/SIDS protein (GST tag N-Terminus) (AB158721)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Iduronate 2 sulfatase/SIDS protein (GST tag N-Terminus) (AB158721)

ab158721 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

P22304

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Cell Lines & Lysates

AB264747

Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell line

Sanger Sequencing - Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell line (AB264747)

  • 0
  • 0
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 61
Cell Lines & Lysates

AB258462

Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell lysate

Sanger Sequencing - Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell lysate (AB258462)

  • 0
  • 0
Primary Antibodies

AB314483

Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-194] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-194] - BSA and Azide free (AB314483)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Iduronate 2 sulfatase.
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Sequence info

[{"sequence":"MPPPRTGRGLLWLGLVLSSVCVALGSETQANSTTDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQEDQSSTGFRLKTSSTRKYK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":312,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P22304","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Iduronate 2-sulfatase also known as IDS or iduronate 2-sulfatase protein is an enzyme responsible for hydrolyzing the 2-sulfate groups from the sugar iduronate in glycosaminoglycans (GAGs) such as dermatan sulfate and heparan sulfate. This action is necessary for the lysosomal degradation of GAGs. The IDS protein has a molecular mass of approximately 76 kDa and predominantly resides in lysosomes. It is widely expressed across human tissues but shows higher expression levels in cells involved with significant lysosomal activities.
Biological function summary

IDS functions to ensure proper breakdown and recycling of GAGs preventing their excessive accumulation. Although IDS acts independently its proper functioning complements other enzymes within the lysosomal degradation system. This lysosomal catabolic activity helps maintain cellular homeostasis as GAGs need controlled degradation for effective cell and tissue functions.

Pathways

IDS plays an important role in the lysosomal degradation pathway. By participating in the catabolism of GAGs IDS works alongside other enzymes such as alpha-L-iduronidase. This interaction is part of the larger lysosomal storage and cellular turnover pathways. These pathways are essential for cellular regulation and help prevent the buildup of undegraded molecules that can disrupt cellular functions.

Faulty IDS activity leads to mucopolysaccharidosis type II also known as Hunter syndrome. This disorder arises from defective GAG breakdown resulting in their accumulation causing various systemic symptoms. IDS is closely related to other lysosomal storage disorder proteins like arylsulfatase B as both enzymes collaborate in GAG degradation. Proper comprehension of IDS and its functioning aids in understanding therapeutic approaches to managing associated disorders.
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Specifications

Form

Liquid

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General info

Function

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.

Sequence similarities

Belongs to the sulfatase family.

Post-translational modifications

Synthesized as a 75-kDa precursor form in the endoplasmic reticulum (ER), and then processed by proteolytic cleavage through various intermediates to yield a 55-kDa mature form, with the release of an 18 kDa polypeptide.. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.
See full target information IDS
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