Recombinant Human Iduronate 2 sulfatase/SIDS protein (His tag) is a Human Full Length protein, in the 26 to 550 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S E T Q A N S T T D A L N V L L I I V D D L R P S L G C Y G D K L V R S P N I D Q L A S H S L L F Q N A F A Q Q A V C A P S R V S F L T G R R P D T T R L Y D F N S Y W R V H A G N F S T I P Q Y F K E N G Y V T M S V G K V F H P G I S S N H T D D S P Y S W S F P P Y H P S S E K Y E N T K T C R G P D G E L H A N L L C P V D V L D V P E G T L P D K Q S T E Q A I Q L L E K M K T S A S P F F L A V G Y H K P H I P F R Y P K E F Q K L Y P L E N I T L A P D P E V P D G L P P V A Y N P W M D I R Q R E D V Q A L N I S V P Y G P I P V D F Q R K I R Q S Y F A S V S Y L D T Q V G R L L S A L D D L Q L A N S T I I A F T S D H G W A L G E H G E W A K Y S N F D V A T H V P L I F Y V P G R T A S L P E A G E K L F P Y L D P F D S A S Q L M E P G R Q S M D L V E L V S L F P T L A G L A G L Q V P P R C P V P S F H V E L C R E G K N L L K H F R F R D L E E D P Y L P G N P R E L I A Y S Q Y P R P S D I P Q W N S D K P S L K D I K I M G Y S I R T I D Y R Y T V W V G F N P D E F L A N F S D I H A G E L Y F V D S D P L Q D H N M Y N D S Q G G D L F Q L L M P L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.
Alternative names
SIDS, IDS, Iduronate 2-sulfatase, Alpha-L-iduronate sulfate sulfatase, Idursulfase
Recommended products
Recombinant Human Iduronate 2 sulfatase/SIDS protein (His tag) is a Human Full Length protein, in the 26 to 550 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P22304-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- S E T Q A N S T T D A L N V L L I I V D D L R P S L G C Y G D K L V R S P N I D Q L A S H S L L F Q N A F A Q Q A V C A P S R V S F L T G R R P D T T R L Y D F N S Y W R V H A G N F S T I P Q Y F K E N G Y V T M S V G K V F H P G I S S N H T D D S P Y S W S F P P Y H P S S E K Y E N T K T C R G P D G E L H A N L L C P V D V L D V P E G T L P D K Q S T E Q A I Q L L E K M K T S A S P F F L A V G Y H K P H I P F R Y P K E F Q K L Y P L E N I T L A P D P E V P D G L P P V A Y N P W M D I R Q R E D V Q A L N I S V P Y G P I P V D F Q R K I R Q S Y F A S V S Y L D T Q V G R L L S A L D D L Q L A N S T I I A F T S D H G W A L G E H G E W A K Y S N F D V A T H V P L I F Y V P G R T A S L P E A G E K L F P Y L D P F D S A S Q L M E P G R Q S M D L V E L V S L F P T L A G L A G L Q V P P R C P V P S F H V E L C R E G K N L L K H F R F R D L E E D P Y L P G N P R E L I A Y S Q Y P R P S D I P Q W N S D K P S L K D I K I M G Y S I R T I D Y R Y T V W V G F N P D E F L A N F S D I H A G E L Y F V D S D P L Q D H N M Y N D S Q G G D L F Q L L M P L E H H H H H H
- Accession
- P22304
- Protein length
- Full Length
- Predicted molecular weight
- 60.3 kDa
- Amino acids
- 26 to 550
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.
- Sequence similarities
Belongs to the sulfatase family.
- Post-translational modifications
Synthesized as a 75-kDa precursor form in the endoplasmic reticulum (ER), and then processed by proteolytic cleavage through various intermediates to yield a 55-kDa mature form, with the release of an 18 kDa polypeptide.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Iduronate 2 sulfatase
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Iduronate 2-sulfatase also known as IDS or iduronate 2-sulfatase protein is an enzyme responsible for hydrolyzing the 2-sulfate groups from the sugar iduronate in glycosaminoglycans (GAGs) such as dermatan sulfate and heparan sulfate. This action is necessary for the lysosomal degradation of GAGs. The IDS protein has a molecular mass of approximately 76 kDa and predominantly resides in lysosomes. It is widely expressed across human tissues but shows higher expression levels in cells involved with significant lysosomal activities.
- Biological function summary
IDS functions to ensure proper breakdown and recycling of GAGs preventing their excessive accumulation. Although IDS acts independently its proper functioning complements other enzymes within the lysosomal degradation system. This lysosomal catabolic activity helps maintain cellular homeostasis as GAGs need controlled degradation for effective cell and tissue functions.
- Pathways
IDS plays an important role in the lysosomal degradation pathway. By participating in the catabolism of GAGs IDS works alongside other enzymes such as alpha-L-iduronidase. This interaction is part of the larger lysosomal storage and cellular turnover pathways. These pathways are essential for cellular regulation and help prevent the buildup of undegraded molecules that can disrupt cellular functions.
- Associated diseases and disorders
Faulty IDS activity leads to mucopolysaccharidosis type II also known as Hunter syndrome. This disorder arises from defective GAG breakdown resulting in their accumulation causing various systemic symptoms. IDS is closely related to other lysosomal storage disorder proteins like arylsulfatase B as both enzymes collaborate in GAG degradation. Proper comprehension of IDS and its functioning aids in understanding therapeutic approaches to managing associated disorders.
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1 product image
SDS-PAGE - Recombinant Human Iduronate 2 sulfatase/SIDS protein (His tag) (ab219294)
15% SDS-PAGE analysis of 3 μg ab219294.
Molecular Weight: 50-70 kDa (SDS-PAGE under reducing conditions).
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