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AB156719

Recombinant Human IEX1/IER3 protein

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Recombinant Human IEX1/IER3 protein is a Human Fragment protein, in the 1 to 82 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DIF2, IEX1, PRG1, IER3, Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Immediate early protein GLY96, Immediate early response 3 protein, PACAP-responsive gene 1 protein, Protein DIF-2, Protein PRG1

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SDS-PAGE - Recombinant Human IEX1/IER3 protein (AB156719)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IEX1/IER3 protein (AB156719)

15% SDS-PAGE analysis of ab156719 (3µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P46695

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as IEX1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMCHSRSCHPTMTILQAPTPAPSTIPGPRRGSGPEIFTFDPLPEPAAAPAGRPSASRGHRKRSRRVLYPRVVRRQLPVEEPNP","proteinLength":"Fragment","predictedMolecularWeight":"11.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":82,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46695","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Immediate Early Response 3 (IER3) also known as IEX1 is an early-response gene product with a mass of approximately 19 kDa. It expresses in various tissues including the skin liver and immune system cells notably in T lymphocytes. IER3 regulates cellular processes such as growth and differentiation by influencing cellular apoptosis and survival mechanisms.
Biological function summary

Immediate Early Response 3 plays a significant role in cellular stress response and immune signaling. IER3 does not form a traditional complex but interacts with intracellular signaling molecules to modulate apoptosis. It also influences the activity of MAPK and NF-kB pathways critical for stress response and inflammation. Through this modulation IER3 directly impacts cell fate under stress conditions.

Pathways

Immediate Early Response 3 is involved in modulating cellular survival and stress response pathways. It interacts with the MAPK signaling pathway which regulates cell growth division and stress response. IER3 influences pathways involving NF-kB a protein complex controlling transcription of DNA and cytokine production. These pathways connect IER3 to other proteins such as Bcl-2 and caspases modifying cellular responses.

Immediate Early Response 3 associates with cancer progression and autoimmune diseases. Its expression levels can influence the development of certain cancers by altering apoptosis and cell proliferation rates. In autoimmune disorders IER3 affects immune responses being linked to proteins like NF-kB which regulates inflammation. Understanding the role of IER3 could aid in developing therapies for conditions like rheumatoid arthritis or certain cancer types.
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Specifications

Form

Liquid

Additional notes

ab156719 is purified using conventional chromatography techniques.

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General info

Function

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.

Sequence similarities

Belongs to the IER3 family.

Post-translational modifications

Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.. Glycosylated.

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Product protocols

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Target data

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
See full target information IER3
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Complementary Products

Primary Antibodies

AB65152

Anti-IEX1/IER3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IEX1/IER3 antibody (AB65152)

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