Recombinant human IFNAR2 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 243 aa range, expressed in HEK 293 and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M L L S Q N A F I F R S L N L V L M V Y I S L V F G I S Y D S P D Y T D E S C T F K I S L R N F R S I L S W E L K N H S I V P T H Y T L L Y T I M S K P E D L K V V K N C A N T T R S F C D L T D E W R S T H E A Y V T V L E G F S G N T T L F S C S H N F W L A I D M S F E P P E F E I V G F T N H I N V M V K F P S I V E E E L Q F D L S L V I E E Q S E G I V K K H K P E I K G N M S G N F T Y I I D K L I P N T N Y C V S V Y L E H S D E Q A V I K S P L K C T L L P P G Q E S E S A E S A K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Together with IFNAR1, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa) (PubMed:10049744, PubMed:10556041, PubMed:21854986, PubMed:26424569, PubMed:28165510, PubMed:32972995, PubMed:7665574, PubMed:7759950, PubMed:8181059, PubMed:8798579, PubMed:8969169). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response (PubMed:10049744, PubMed:17517919, PubMed:21854986, PubMed:26424569, PubMed:28165510, PubMed:32972995, PubMed:7665574, PubMed:7759950, PubMed:8181059, PubMed:8798579, PubMed:8969169). Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another (PubMed:10556041, PubMed:11682488, PubMed:12105218, PubMed:21854986, PubMed:32972995). The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (STAT1, STAT2 and STAT) (PubMed:11682488, PubMed:12105218, PubMed:21854986, PubMed:32972995). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes (PubMed:12105218, PubMed:28165510, PubMed:9121453). Isoform 3. Potent inhibitor of type I IFN receptor activity.
Alternative names
IFNABR, IFNARB, IFNAR2, Interferon alpha/beta receptor 2, IFN-R-2, IFN-alpha binding protein, IFN-alpha/beta receptor 2, Interferon alpha binding protein, Type I interferon receptor 2
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Recombinant human IFNAR2 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 243 aa range, expressed in HEK 293 and suitable for SDS-PAGE, FuncS.
Key facts
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized PPICZa-human IFNA2 recombinant protein at 10 μg/mL (100 μL/well) can bind huamn IFNAR2 recombinant protein (hFc Tag), the EC50 of IFNaR2-Fc is 60-200 ng/mL.
- Accession
- P48551-1
- Animal free
- No
- Species
- Human
- Concentration
Sequence info
Amino acid sequence
- M L L S Q N A F I F R S L N L V L M V Y I S L V F G I S Y D S P D Y T D E S C T F K I S L R N F R S I L S W E L K N H S I V P T H Y T L L Y T I M S K P E D L K V V K N C A N T T R S F C D L T D E W R S T H E A Y V T V L E G F S G N T T L F S C S H N F W L A I D M S F E P P E F E I V G F T N H I N V M V K F P S I V E E E L Q F D L S L V I E E Q S E G I V K K H K P E I K G N M S G N F T Y I I D K L I P N T N Y C V S V Y L E H S D E Q A V I K S P L K C T L L P P G Q E S E S A E S A K
- Accession
- P48551
- Protein length
- Fragment
- Predicted molecular weight
- 51.8 kDa
- Amino acids
- 1 to 243
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
General info
- Function
Together with IFNAR1, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa) (PubMed:10049744, PubMed:10556041, PubMed:21854986, PubMed:26424569, PubMed:28165510, PubMed:32972995, PubMed:7665574, PubMed:7759950, PubMed:8181059, PubMed:8798579, PubMed:8969169). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response (PubMed:10049744, PubMed:17517919, PubMed:21854986, PubMed:26424569, PubMed:28165510, PubMed:32972995, PubMed:7665574, PubMed:7759950, PubMed:8181059, PubMed:8798579, PubMed:8969169). Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another (PubMed:10556041, PubMed:11682488, PubMed:12105218, PubMed:21854986, PubMed:32972995). The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (STAT1, STAT2 and STAT) (PubMed:11682488, PubMed:12105218, PubMed:21854986, PubMed:32972995). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes (PubMed:12105218, PubMed:28165510, PubMed:9121453).
- Sequence similarities
Belongs to the type II cytokine receptor family.
- Post-translational modifications
Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFNAR2 also known as Interferon Alpha/Beta Receptor 2 is a critical part of the immune system. It pairs with IFNAR1 to form the receptor complex binding type I interferons such as IFN-alpha and IFN-beta. The IFNAR2 protein itself weighs roughly 60 kDa. You can find it expressed on the surface of almost all cell types making it a versatile participant in cellular immune responses. Cellular assays such as IFN-alpha ELISA often use IFNAR2 to quantify type I interferon activity and researchers frequently study it to understand its role in immune regulation.
- Biological function summary
IFNAR2 plays a role in mediating the effects of type I interferons. It forms part of a receptor complex with IFNAR1 to initiate signal transduction upon binding to its ligands leading to activation of the JAK-STAT signaling pathway. This pathway then induces the expression of interferon-stimulated genes (ISGs) that promote antiviral defense cell growth control and modulation of the immune response. Without the function of IFNAR2 within this complex cells would be unable to respond appropriately to viral infections.
- Pathways
IFNAR2 is widely recognized in its involvement with the JAK-STAT pathway. Through this pathway IFNAR2 interacts closely with various proteins like JAK1 and STAT1 propagating the signal that leads to the transcription of numerous ISGs. Another vital pathway involving IFNAR2 is the induction of cytokines which influences the adaptive immune response. Its position within these pathways illustrates its functional importance in antiviral defense and immune system regulation.
- Associated diseases and disorders
IFNAR2 has associations with multiple sclerosis and systemic lupus erythematosus. In multiple sclerosis type I interferon pathways mediated by IFNAR2 can modulate immune responses implicated in the progression of this neurodegenerative disorder. Increased IFNAR2 activity might exacerbate lupus characterized by improper immune system regulation. IFN-alpha a cytokine closely linked to IFNAR2 plays a pivotal role in these conditions highlighting its importance in disease development and potential therapeutic targeting.
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2 product images
Functional Studies - Recombinant human IFNAR2 protein (Fc Chimera) (ab276261)
Measured by its binding ability in a functional ELISA. Immobilized PPICZa-human IFNA2 recombinant protein at 10 μg/mL (100 μL/well) can bind huamn IFNAR2 recombinant protein (hFc Tag), the EC50 of IFNaR2-Fc is 60-200 ng/mL.
SDS-PAGE - Recombinant human IFNAR2 protein (Fc Chimera) (ab276261)
SDS-PAGE analysis of ab276261.
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