Recombinant human IFNW1 protein (His tag) is a Human Full Length protein, in the 1 to 195 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A L L F P L L A A L V M T S Y S P V G S L G C D L P Q N H G L L S R N T L V L L H Q M R R I S P F L C L K D R R D F R F P Q E M V K G S Q L Q K A H V M S V L H E M L Q Q I F S L F H T E R S S A A W N M T L L D Q L H T G L H Q Q L Q H L E T C L L Q V V G E G E S A G A I S S P A L T L R R Y F Q G I R V Y L K E K K Y S D C A W E V V R M E I M K S L F L S T N M Q E R L R S K D R D L G S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Interferon omega-1, Interferon alpha-II-1, IFNW1
Recommended products
Recombinant human IFNW1 protein (His tag) is a Human Full Length protein, in the 1 to 195 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV).
The ED50 for this effect is typically 2-8 pg/mL. - Accession
- P05000-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A L L F P L L A A L V M T S Y S P V G S L G C D L P Q N H G L L S R N T L V L L H Q M R R I S P F L C L K D R R D F R F P Q E M V K G S Q L Q K A H V M S V L H E M L Q Q I F S L F H T E R S S A A W N M T L L D Q L H T G L H Q Q L Q H L E T C L L Q V V G E G E S A G A I S S P A L T L R R Y F Q G I R V Y L K E K K Y S D C A W E V V R M E I M K S L F L S T N M Q E R L R S K D R D L G S S
- Accession
- P05000
- Protein length
- Full Length
- Predicted molecular weight
- 21.6 kDa
- Amino acids
- 1 to 195
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Sequence similarities
Belongs to the alpha/beta interferon family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFNW1 also known as interferon omega is a type I interferon with a mass of approximately 23 kDa. Mechanically this protein functions in the immune response by inducing antiviral state in cells. It is expressed in various tissues including immune cells like leukocytes. IFNW1 binds to the specific interferon alpha/beta receptor (IFNAR) to exert its effects. This interaction triggers signaling cascades that activate the transcription of interferon-stimulated genes which are critical in antiviral defense.
- Biological function summary
IFNW1 plays important roles in modulating the immune response and controlling viral infections. It is not part of a known protein complex but interacts with IFNAR to activate signaling pathways. It enhances the expression of antiviral proteins and cells' ability to combat pathogen intrusions. Beyond antiviral activity IFNW1 can influence cell differentiation and the immune system's surveillance capabilities ensuring its response against threats is effective.
- Pathways
IFNW1 contributes to the JAK-STAT signaling pathway and the innate immune response. In the JAK-STAT pathway it activates key proteins such as JAK1 and TYK2 which lead to phosphorylation and activation of STAT proteins. These activated STAT proteins then translocate to the nucleus and promote transcription of genes involved in antiviral defense. IFNW1 shares pathways with other interferons like IFNA and IFNB making it an integral part of the cytokine signaling network that regulates immune function.
- Associated diseases and disorders
IFNW1 has associations with viral infections and autoimmune disorders like systemic lupus erythematosus (SLE). Its role in antiviral defense positions IFNW1 as a target for understanding virus-host interactions that contribute to pathogenesis. In autoimmune disorders like SLE the dysregulation of interferon signaling including that involving IFNW1 links to the aberrant immune activation seen in these conditions. IFNW1's interactions with IFNA and IFNB highlight its involvement in pathways that can drive disease states emphasizing its relevance in disease research and potential therapeutic approaches.
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2 product images
Functional Studies - Recombinant human IFNW1 protein (His tag) (ab276259)
Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV).
The ED50 for this effect is typically 2-8 pg/mL.
SDS-PAGE - Recombinant human IFNW1 protein (His tag) (ab276259)
SDS-PAGE analysis of ab276259
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