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Recombinant human IGF1 protein (Active) is a Human Full Length protein, in the 49 to 118 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biologically active

Yes

Sequence

G P E T L C G A E L V D A L Q F V C G D R G F Y F N K P T G Y G S S S R R A P Q T G I V D E C C F R S C D L R R L E M Y C A P L K P A K S A

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human IGF1 protein (Active) is a Human Full Length protein, in the 49 to 118 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biological activity

Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells.

ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.

Predicted molecular weight

7.71 kDa

Amino acids

49 to 118

Sequence

G P E T L C G A E L V D A L Q F V C G D R G F Y F N K P T G Y G S S S R R A P Q T G I V D E C C F R S C D L R R L E M Y C A P L K P A K S A

Accession
P05019-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

Purity by HPLC >=95%.

General info

Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23696648, PubMed:23243309).

Sequence similarities

Belongs to the insulin family.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilized prior to aliquoting and lyophilization. All aliquoting and lyophilization steps are performed in a sterile environment

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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