Recombinant human IGF1 protein (Active) is a Human Full Length protein in the 49 to 118 aa range with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, Cell Culture, mass spectrometry and HPLC. The predicted molecular weight of ab270062 protein is 7.7 kDa.
- Save time and ensure accurate results - use our human Insulin-like growth factor I (IGF-1) protein as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
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- Stem cell research & therapy 15:42024Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Luo,Kaiwen Wu,Xiaolong Zhang,Hongyang Wang,Qiuju WangPubMed 38167128
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229352023MiR-24-3p regulates the differentiation of adipose-derived stem cells toward pericytes and promotes fat grafting vascularization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongming Cai,Zihao Li,Qing Wei,Fangfang Yang,Tian Li,Chen Ke,Yucang He,Jingping Wang,Binting Ni,Ming Lin,Liqun LiPubMed 37086094
- Veterinary research communications 47:1523-15332023The effects of apelin on IGF1/FSH-induced steroidogenesis, proliferation, Bax expression, and total antioxidant capacity in granulosa cells of buffalo ovarian follicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBorhan Shokrollahi,Hai-Ying Zheng,Xiao-Ya Ma,Jiang-Hua ShangPubMed 37036601
- Cells 12:2023Phosphorylation of IGFBP-3 by Casein Kinase 2 Blocks Its Interaction with Hyaluronan, Enabling HA-CD44 Signaling Leading to Increased NSCLC Cell Survival and Cisplatin Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-Ling Coleman,Michael Chiaramonti,Ben Haddad,Robert Ranzenberger,Heather Henning,Hind Al Khashali,Ravel Ray,Ban Darweesh,Jeffrey Guthrie,Deborah Heyl,Hedeel Guy EvansPubMed 36766747
- Nature communications 13:55002022Structure of the PAPP-A complex reveals mechanism of substrate recognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRussell A Judge,Janani Sridar,Kathryn Tunyasuvunakool,Rinku Jain,John C K Wang,Christna Ouch,Jun Xu,Amirhossein Mafi,Aaron H Nile,Clint Remarcik,Corey L Smith,Crystal Ghosh,Chen Xu,Vincent Stoll,John Jumper,Amoolya H Singh,Dan Eaton,Qi HaoPubMed 36127359
- Journal of nanobiotechnology 20:3152022IGF1 receptor-targeted black TiO nanoprobes for MRI-guided synergetic photothermal-chemotherapy in drug resistant pancreatic tumor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiwei Xu,Lufei Jin,Liu Xu,Yuchao Zhu,Lu Hong,Chunshu Pan,Yanying Li,Junlie Yao,Ruifen Zou,Weiwei Tang,Jianhua Wang,Aiguo Wu,Wenzhi RenPubMed 35794573
- Journal of cellular and molecular medicine 26:2881-28942022Effect of Glut-1 and HIF-1α double knockout by CRISPR/CAS9 on radiosensitivity in laryngeal carcinoma via the PI3K/Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang-Yang Bao,Jiang-Tao Zhong,Li-Fang Shen,Li-Bo Dai,Shui-Hong Zhou,Jun Fan,Hong-Tian Yao,Zhong-Jie LuPubMed 35415942
- Frontiers in endocrinology 13:8443602022Apelin and Apelin Receptor in Follicular Granulosa Cells of Buffalo Ovaries: Expression and Regulation of Steroidogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBorhan Shokrollahi,Hai-Ying Zheng,Ling-Yu Li,Li-Ping Tang,Xiao-Ya Ma,Xing-Rong Lu,An-Qin Duan,Yu Zhang,Xiao-Hui Tan,Chen-Xi Huang,Yuan-Yuan Xu,Jiang-Hua ShangPubMed 35355567
- Cancer cell international 22:132022Hypoxia-mediated YTHDF2 overexpression promotes lung squamous cell carcinoma progression by activation of the mTOR/AKT axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Xu,Kang Hu,Ping Zhang,Zhi-Gang Sun,Nan ZhangPubMed 34996459
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
Amino acid sequence
G P E T L C G A E L V D A L Q F V C G D R G F Y F N K P T G Y G S S S R R A P Q T G I V D E C C F R S C D L R R L E M Y C A P L K P A K S A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23243309, PubMed:23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
Alternative names
IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF
Recommended products
Recombinant human IGF1 protein (Active) is a Human Full Length protein in the 49 to 118 aa range with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, Cell Culture, mass spectrometry and HPLC. The predicted molecular weight of ab270062 protein is 7.7 kDa.
- Save time and ensure accurate results - use our human Insulin-like growth factor I (IGF-1) protein as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells.
ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.
- Accession
- P05019-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- G P E T L C G A E L V D A L Q F V C G D R G F Y F N K P T G Y G S S S R R A P Q T G I V D E C C F R S C D L R R L E M Y C A P L K P A K S A
- Accession
- P05019
- Protein length
- Full Length
- Predicted molecular weight
- 7.71 kDa
- Amino acids
- 49 to 118
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23243309, PubMed:23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
- Sequence similarities
Belongs to the insulin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our recombinant human IGF1 protein ab270062 as a control.
The ab270062 IGF-1 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.
Check out our protein gel staining guide for SDS-PAGE here
Premium Bioactive Protein range
The ab270062 IGF-1 protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
More premium bioactive proteins can be found here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
- Biological function summary
IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.
- Pathways
IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.
- Associated diseases and disorders
Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
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4 product images
Functional Studies - Recombinant human IGF1 protein (Active) (ab270062)
Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells. The ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.
SDS-PAGE - Recombinant human IGF1 protein (Active) (ab270062)
SDS-PAGE analysis of ab270062.
HPLC - Recombinant human IGF1 protein (Active) (ab270062)
Purity ≥95%
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Mass Spectrometry - Recombinant human IGF1 protein (Active) (ab270062)
M - 2.84 Da (Calculated mass 7711.84 Da)
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
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