Recombinant Human IGF2 protein (Active) is a Human Fragment protein, in the 25 to 91 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC, Biological Activity, Cell Culture.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC, Biological Activity, Cell Culture
- Biologically active
- Yes
Amino acid sequence
A Y R P S E T L C G G E L V D T L Q F V C G D R G F Y F S R P A S R V S R R S R G I V E E C C F R S C D L A L L E T Y C A T P A K S E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Biological Activity | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The insulin-like growth factors possess growth-promoting activity (By similarity). Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling (PubMed:28873464). Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate (By similarity). Preptin undergoes glucose-mediated co-secretion with insulin, and acts as a physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Alternative names
IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II
Recommended products
Recombinant Human IGF2 protein (Active) is a Human Fragment protein, in the 25 to 91 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC, Biological Activity, Cell Culture.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC, Biological Activity, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent proliferation of MCF7 cells. ED50 is ≤5.8 ng/mL, corresponding to a specific activity of 1.72 x 105 units/mg.
- Accession
- P01344-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A Y R P S E T L C G G E L V D T L Q F V C G D R G F Y F S R P A S R V S R R S R G I V E E C C F R S C D L A L L E T Y C A T P A K S E
- Accession
- P01344
- Protein length
- Fragment
- Predicted molecular weight
- 20 kDa
- Amino acids
- 25 to 91
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
The insulin-like growth factors possess growth-promoting activity (By similarity). Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling (PubMed:28873464). Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate (By similarity).
- Sequence similarities
Belongs to the insulin family.
- Post-translational modifications
O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Biological Activity - Recombinant Human IGF2 protein (Active) (ab283420)
Functional analysis of ab283420.
Fully biologically active determined by the dose dependent proliferation of MCF7 cells. ED50 is ≤5.8 ng/mL, corresponding to a specific activity of 1.72 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3430138-2
SDS-PAGE - Recombinant Human IGF2 protein (Active) (ab283420)
SDS-PAGE analysis of ab283420.
Mass Spectrometry - Recombinant Human IGF2 protein (Active) (ab283420)
Mass determination by ESI-TOF.
Predicted MW is 7532.52 (+/- 10Da by ESI-TOF). MW observed 7532.89.
HPLC - Recombinant Human IGF2 protein (Active) (ab283420)
HPLC analysis of ab283420.
Functional Studies - Recombinant Human IGF2 protein (Active) (ab283420)
Fully biologically active determined by dose dependent proliferation of MCF7 cell. ED50 is ≤ 5.8 ng/ml, corresponding to a specific activity of 1.72x10^5 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3430138-1
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