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AB280941

Recombinant human IGFBP3 protein (Active)

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(3 Publications)

Recombinant human IGFBP3 protein (Active) is a Human Full Length protein, in the 28 to 291 aa range, expressed in HEK 293T cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

4 Images
Mass Spectrometry - Recombinant human IGFBP3 protein (Active) (AB280941)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human IGFBP3 protein (Active) (AB280941)

Mass determination by ESI-TOF.

Predicted MW is 28630.43 Da. (+/- 10 Da by ESI-TOF). Observes MW is 28633.14 Da. Additional masses at 29201.30 is due to residual O-glycans.

Functional Studies - Recombinant human IGFBP3 protein (Active) (AB280941)
  • FuncS

Supplier Data

Functional Studies - Recombinant human IGFBP3 protein (Active) (AB280941)

Fully biologically active determined by the dose dependent decrease in MCF7 cell proliferation.

ED50 is 38.33 ng/ml, corresponding to a specific activity of 0.26 x 105 units /mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3381125-1

HPLC - Recombinant human IGFBP3 protein (Active) (AB280941)
  • HPLC

Supplier Data

HPLC - Recombinant human IGFBP3 protein (Active) (AB280941)

HPLC analysis of ab280941.

SDS-PAGE - Recombinant human IGFBP3 protein (Active) (AB280941)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IGFBP3 protein (Active) (AB280941)

SDS-PAGE analysis of ab280941.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293T cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, HPLC, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent decrease in MCF7 cell proliferation.

ED50 is 38.33 ng/ml, corresponding to a specific activity of 0.26 x 105 units /mg.

Accession

P17936

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"GGASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK","proteinLength":"Full Length","predictedMolecularWeight":"28.63 kDa","actualMolecularWeight":"28.63 kDa","aminoAcidEnd":291,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293T cells","accessionNumber":"P17936","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

>=95% Purity by HPLC

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General info

Function

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 10874028, PubMed : 19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed : 20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed : 19623253). Promotes testicular germ cell apoptosis (PubMed : 19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed : 9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed : 15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed : 10874028).

Post-translational modifications

Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2; resulting in decreased nuclear localization (PubMed:19556345).

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Product protocols

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 10874028, PubMed : 19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed : 20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed : 19623253). Promotes testicular germ cell apoptosis (PubMed : 19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed : 9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed : 15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed : 10874028).
See full target information IGFBP3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 15:263 PubMed39183355

2024

Discovering genes and microRNAs involved in human lung development unveils IGFBP3/miR-34a dynamics and their relevance for alveolar differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Acosta-Plasencia,Joan J Castellano,Tania Díaz,Yangyi He,Ramón M Marrades,Alfons Navarro

Communications chemistry 6:234 PubMed37898658

2023

Cryo-EM structure of human PAPP-A2 and mechanism of substrate recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Janani Sridar,Amirhossein Mafi,Russell A Judge,Jun Xu,Kailyn A Kong,John C K Wang,Vincent S Stoll,Georgios Koukos,Reyna J Simon,Dan Eaton,Matthew Bratkowski,Qi Hao

Scientific reports 12:11503 PubMed35798794

2022

Roles of IGFBP-3 in cell migration and growth in an endophytic tongue squamous cell carcinoma cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Feng Ying Ng,Atsushi Kaida,Hitomi Nojima,Masahiko Miura
View all publications
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