Recombinant human IGFBP3 protein (Active) is a Human Full Length protein, in the 28 to 291 aa range, expressed in HEK 293T, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
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- Communications chemistry 6:2342023Cryo-EM structure of human PAPP-A2 and mechanism of substrate recognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanani Sridar,Amirhossein Mafi,Russell A Judge,Jun Xu,Kailyn A Kong,John C K Wang,Vincent S Stoll,Georgios Koukos,Reyna J Simon,Dan Eaton,Matthew Bratkowski,Qi HaoPubMed 37898658
- Scientific reports 12:115032022Roles of IGFBP-3 in cell migration and growth in an endophytic tongue squamous cell carcinoma cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsther Feng Ying Ng,Atsushi Kaida,Hitomi Nojima,Masahiko MiuraPubMed 35798794
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293T cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
G G A S S A G L G P V V R C E P C D A R A L A Q C A P P P A V C A E L V R E P G C G C C L T C A L S E G Q P C G I Y T E R C G S G L R C Q P S P D E A R P L Q A L L D G R G L C V N A S A V S R L R A Y L L P A P P A P G N A S E S E E D R S A G S V E S P S V S S T H R V S D P K F H P L H S K I I I I K K G H A K D S Q R Y K V D Y E S Q S T D T Q N F S S E S K R E T E Y G P C R R E M E D T L N H L K F L N V L S P R G V H I P N C D K K G F Y K K K Q C R P S K G R K R G F C W C V D K Y G Q P L P G Y T T K G K E D V H C Y S M Q S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:10874028, PubMed:19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed:20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253). Promotes testicular germ cell apoptosis (PubMed:19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed:9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed:15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed:10874028).
Alternative names
IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3
Recommended products
Recombinant human IGFBP3 protein (Active) is a Human Full Length protein, in the 28 to 291 aa range, expressed in HEK 293T, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293T cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent decrease in MCF7 cell proliferation.
ED50 is 38.33 ng/ml, corresponding to a specific activity of 0.26 x 105 units /mg.
- Accession
- P17936-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- G G A S S A G L G P V V R C E P C D A R A L A Q C A P P P A V C A E L V R E P G C G C C L T C A L S E G Q P C G I Y T E R C G S G L R C Q P S P D E A R P L Q A L L D G R G L C V N A S A V S R L R A Y L L P A P P A P G N A S E S E E D R S A G S V E S P S V S S T H R V S D P K F H P L H S K I I I I K K G H A K D S Q R Y K V D Y E S Q S T D T Q N F S S E S K R E T E Y G P C R R E M E D T L N H L K F L N V L S P R G V H I P N C D K K G F Y K K K Q C R P S K G R K R G F C W C V D K Y G Q P L P G Y T T K G K E D V H C Y S M Q S K
- Accession
- P17936
- Protein length
- Full Length
- Predicted molecular weight
- 28.63 kDa
- Actual molecular weight
- 28.63 kDa
- Amino acids
- 28 to 291
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>=95% Purity by HPLC
General info
- Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:10874028, PubMed:19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed:20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253). Promotes testicular germ cell apoptosis (PubMed:19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed:9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed:15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed:10874028).
- Post-translational modifications
Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2; resulting in decreased nuclear localization (PubMed:19556345).
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant human IGFBP3 protein (Active) (ab280941)
Fully biologically active determined by the dose dependent decrease in MCF7 cell proliferation.
ED50 is 38.33 ng/ml, corresponding to a specific activity of 0.26 x 105 units /mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3381125-1
SDS-PAGE - Recombinant human IGFBP3 protein (Active) (ab280941)
SDS-PAGE analysis of ab280941.
HPLC - Recombinant human IGFBP3 protein (Active) (ab280941)
HPLC analysis of ab280941.
Mass Spectrometry - Recombinant human IGFBP3 protein (Active) (ab280941)
Mass determination by ESI-TOF.
Predicted MW is 28630.43 Da. (+/- 10 Da by ESI-TOF). Observes MW is 28633.14 Da. Additional masses at 29201.30 is due to residual O-glycans.
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