Recombinant Human IGFBP5 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS, WB, Inhib.
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- Breast cancer research : BCR 21:562019Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Slocum,Amanda Craig,Augusto Villanueva,Doris GermainPubMed 31046834
- The Journal of biological chemistry 285:12925-342010Insulin-like growth factor-binding protein-5-induced laminin gamma1 transcription requires filamin A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristine K Abrass et. al.PubMed 20167606
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB, Inhib
- Biologically active
- No
Amino acid sequence
M L G S F V H C E P C D E K A L S M C P P S P L G C E L V K E P G C G C C M T C A L A E G Q S C G V Y T E R C A Q G L R C L P R Q D E E K P L H A L L H G R G V C L N E K S Y R E Q V K I E R D S R E H E E P T T S E M A E E T Y S P K I F R P K H T R I S E L K A E A V K K D R R K K L T Q S K F V G G A E N T A H P R I I S A P E M R Q E S E Q G P C R R H M E A S L Q E L K A S P R M V P R A V Y L P N C D R K G F Y K R K Q C K P S R G R K R G I C W C V D K Y G M K L P G M E Y V D G D F Q C H T F D S S N V S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab49835 can be used as a WB positive control in conjunction with ab84229. |
Application Inhib | Reactivity Reacts | Dilution info 0.3 µg/mL | Notes The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15 ng/ml of human IGF-II. |
Target data
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:18930415, PubMed:7683690). Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells (By similarity). Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling (By similarity). Plays also a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs (PubMed:20345844, PubMed:26103640). Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling (PubMed:36949068).
Alternative names
IBP5, IGFBP5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5
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Recombinant Human IGFBP5 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS, WB, Inhib.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB, Inhib
- Biologically active
- No
- Accession
- P24593-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
- Concentration
- Storage buffer
Constituents: 0.294% Sodium citrate
Sequence info
Amino acid sequence
- M L G S F V H C E P C D E K A L S M C P P S P L G C E L V K E P G C G C C M T C A L A E G Q S C G V Y T E R C A Q G L R C L P R Q D E E K P L H A L L H G R G V C L N E K S Y R E Q V K I E R D S R E H E E P T T S E M A E E T Y S P K I F R P K H T R I S E L K A E A V K K D R R K K L T Q S K F V G G A E N T A H P R I I S A P E M R Q E S E Q G P C R R H M E A S L Q E L K A S P R M V P R A V Y L P N C D R K G F Y K R K Q C K P S R G R K R G I C W C V D K Y G M K L P G M E Y V D G D F Q C H T F D S S N V S
- Accession
- P24593
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Greater than 98% by SDS-PAGE gel and HPLC analyses.
General info
- Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:18930415, PubMed:7683690). Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells (By similarity). Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling (By similarity). Plays also a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs (PubMed:20345844, PubMed:26103640). Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling (PubMed:36949068).
- Post-translational modifications
Cleaved by C1S in extracellular space.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
Western blot - Recombinant Human IGFBP5 protein (ab49835)
All lanes: Anti-IGFBP5 antibody (Biotin) (ab84229) at 0.1 µg/mL
All lanes: Western blot - Recombinant Human IGFBP5 protein (ab49835) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30s
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