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Recombinant Human IgG1 Fc Protein, His Tag (Active) is a Human Fragment protein, in the 99 to 330 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names=Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
Yes
- Sequence
E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes The reducing (R) protein migrates as 30-33 kDa in SDS-PAGE due to glycosylation. |
Images
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
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Recombinant Human IgG1 Fc Protein, His Tag (Active) is a Human Fragment protein, in the 99 to 330 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names=Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
Yes
- Biological activity
Loaded Biotinylated Human CD64, His,Avitag can bind ab219660 with an affinity constant of 1.60 nM as determined in BLI assay.
- Predicted molecular weight
27 kDa
- Amino acids
99 to 330
- Sequence
E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
- Accession
- P01857-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: Glycine, L-Arginine, Sodium chloride, 5% Trehalose, 0.61% Tris
Specifications
- Form
Lyophilized
- Additional notes
Purified by Immobilized metal affinity chromatography.
General info
- Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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