Recombinant Human IgG4 Fc Protein, Tag Free (Active) is a Human Fragment protein, in the 99 to 327 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, SPR.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, SPR
- Biologically active
- Yes
Amino acid sequence
E S K Y G P P C P S C P A P E F L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S Q E D P E V Q F N W Y V D G V E V H N A K T K P R E E Q F N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K G L P S S I E K T I S K A K G Q P R E P Q V Y T L P P S Q E E M T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S R L T V D K S R W Q E G N V F S C S V M H E A L H N H Y T Q K S L S L S L G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application SPR | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
Recommended products
Recombinant Human IgG4 Fc Protein, Tag Free (Active) is a Human Fragment protein, in the 99 to 327 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, SPR.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, SPR
- Biologically active
- Yes
- Biological activity
- Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind ab181968 with an affinity constant of 0.715 μM as determined in SPR assay (Biacore).
- Accession
- P01861-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 500 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 6.8% Trehalose, 0.51% Glycine, 0.41% Tris, 0.36% L-Arginine hydrochloride, 0.04% Sodium chloride
Sequence info
Amino acid sequence
- E S K Y G P P C P S C P A P E F L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S Q E D P E V Q F N W Y V D G V E V H N A K T K P R E E Q F N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K G L P S S I E K T I S K A K G Q P R E P Q V Y T L P P S Q E E M T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S R L T V D K S R W Q E G N V F S C S V M H E A L H N H Y T Q K S L S L S L G K
- Accession
- P01861
- Protein length
- Fragment
- Predicted molecular weight
- 25.8 kDa
- Amino acids
- 99 to 327
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
- Post-translational modifications
Glycosylation on Asn-177 is required for interaction with Fc receptors and ability to activate the complement pathway.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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2 product images
SDS-PAGE - Recombinant Human IgG4 Fc Protein, Tag Free (Active) (ab181968)
SDS-PAGE of DDT-reduced (lane 1) and non-reduced (lane 2) ab181968 stained overnight with Coomassie Blue.
As a result of glycosylation, DTT-reduced (R) protein migrates as 30-32 kDa and non-reduced (NR) protein migrates as 28-30 and 55-60 kDa.
Surface Plasmon Resonance - Recombinant Human IgG4 Fc Protein, Tag Free (Active) (ab181968)
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind ab181968 with an affinity constant of 0.715 μM as determined in SPR assay (Biacore).
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