Recombinant human Ihh protein (Active) is a Human Fragment protein, in the 29 to 202 aa range, expressed in Escherichia coli, with >96% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
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Publications
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- International journal of molecular sciences 21:2020Discrete Hedgehog Factor Expression and Action in the Developing Phallus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGerard A Tarulli et. al.PubMed 32059607
Key facts
- Purity
- >96% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC
- Biologically active
- Yes
Amino acid sequence
I I G P G R V V G S R R R P P R K L V P L A Y K Q F S P N V P E K T L G A S G R Y E G K I A R S S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D R L N S L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R N K Y G L L A R L A V E A G F D W V Y Y E S K A H V H C S V K S E H S A A A K T G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Indian hedgehog protein. The C-terminal part of the indian hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Plays a role in hedgehog paracrine signaling (PubMed:24342078). Associated with the very-low-density lipoprotein (VLDL) particles to function as a circulating morphogen for endothelial cell integrity maintenance (PubMed:20839884). Indian hedgehog protein N-product. The dually lipidated indian hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity). Plays a role in morphogenesis of the skeleton by coordinating growth and differentiation of the endochondral skeleton (By similarity). Positively regulates PTHLH expression during endochondral bone formation preventing chondrocyte hypertrophy. In contrast, participates in normal chondrocyte proliferation in a PTHLH-independent pathway (By similarity).
Alternative names
Indian hedgehog protein, IHH, HHG-2
Recommended products
Recombinant human Ihh protein (Active) is a Human Fragment protein, in the 29 to 202 aa range, expressed in Escherichia coli, with >96% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
Key facts
- Purity
- >96% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.
- Accession
- Q14623-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water or 0.1% BSA aqueous buffer
- Concentration
- Storage buffer
Constituents: Phosphate Buffer, 0.87% Sodium chloride
Sequence info
Amino acid sequence
- I I G P G R V V G S R R R P P R K L V P L A Y K Q F S P N V P E K T L G A S G R Y E G K I A R S S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D R L N S L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R N K Y G L L A R L A V E A G F D W V Y Y E S K A H V H C S V K S E H S A A A K T G G
- Accession
- Q14623
- Protein length
- Fragment
- Predicted molecular weight
- 19.8 kDa
- Amino acids
- 29 to 202
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>96% as determined by HPLC.
General info
- Function
Indian hedgehog protein
- Sequence similarities
Belongs to the hedgehog family.
- Post-translational modifications
Indian hedgehog protein N-product
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Indian hedgehog (Ihh) protein known by its gene symbol IHH plays key roles in cell signaling. Ihh with an approximate mass of 45 kDa belongs to the Hedgehog family of signaling molecules. It is expressed in various tissues including cartilage bone and parts of the gastrointestinal tract where it regulates growth and patterning. Ihh acts by binding to its receptor Patched triggering downstream signaling that influences cell fate decisions.
- Biological function summary
Ihh is significant in regulating the differentiation and proliferation of chondrocytes in the growth plate of long bones. It acts as part of a complex regulatory network coordinating with Parathyroid hormone-related protein (PTHrP) to maintain proper development of endochondral bones. Ihh also influences epithelial and mesenchymal interactions during embryo development evidencing its important role in developmental biology.
- Pathways
Ihh is a critical component of the Hedgehog signaling pathway a conserved pathway involved in embryonic development and adult tissue homeostasis. This pathway also includes proteins like Sonic hedgehog (Shh) and Desert hedgehog (Dhh) as well as the receptor Smoothened which mediates the signaling cascade. Ihh signaling impacts pathways such as the Wnt pathway influencing the proliferation and differentiation of cells thereby highlighting its integration into broader cellular signaling networks.
- Associated diseases and disorders
Ihh misregulation can lead to skeletal disorders like brachydactyly type A1 characterized by shortened fingers and toes. Furthermore abnormal Ihh signaling can contribute to certain cancers such as chondrosarcoma where overactive Hedgehog signaling promotes tumorigenesis. The relationship between Ihh and other Hedgehog proteins including Shh is key in understanding these pathologies as they share pathways that are often disrupted in disease scenarios.
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SDS-PAGE - Recombinant human Ihh protein (Active) (ab243268)
SDS-PAGE analysis of ab243268
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