Recombinant Human Ikaros protein is a Human Full Length protein, in the 1 to 519 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
T7 tag N-Terminus
SDS-PAGE
No
M A S M T G G Q Q M G R G H H H H H H G N L Y F Q G G E F D A D E G Q D M S Q V S G K E S P P V S D T P D E G D E P M P I P E D L S T T S G G Q Q S S K S D R V V A S N V K V E T Q S D E E N G R A C E M N G E E C A E D L R M L D A S G E K M N G S H R D Q G S S A L S G V G G I R L P N G K L K C D I C G I I C I G P N V L M V H K R S H T G E R P F Q C N Q C G A S F T Q K G N L L R H I K L H S G E K P F K C H L C N Y A C R R R D A L T G H L R T H S V G K P H K C G Y C G R S Y K Q R S S L E E H K E R C H N Y L E S M G L P G T L Y P V I K E E T N H S E M A E D L C K I G S E R S L V L D R L A S N V A K R K S S M P Q K F L G D K G L S D T P Y D S S A S Y E K E N E M M K S H V M D Q A I N N A I N Y L G A E S L R P L V Q T P P G G S E V V P V I S P M Y Q L H K P L A E G T P R S N H S A Q D S A V E N L L L L S K A K L V P S E R E A S P S N S C Q D S T D T E S N N E E Q R S G L I Y L T N H I A P H A R N G L S L K E E H R A Y D L L R A A S E N S Q D A L R V V S T S G E Q M K V Y K C E H C R V L F L D H V M Y T I H M G C H G F R D P F E C N M C G Y H S Q D R Y E F S S H I T R G E H R F H M S
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1, IKZF1, ZNFN1A1, LYF1, IK1, IKAROS
Recombinant Human Ikaros protein is a Human Full Length protein, in the 1 to 519 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
T7 tag N-Terminus
SDS-PAGE
No
No
Human
pH: 8
Constituents: 0.32% Tris-HCl buffer
M A S M T G G Q Q M G R G H H H H H H G N L Y F Q G G E F D A D E G Q D M S Q V S G K E S P P V S D T P D E G D E P M P I P E D L S T T S G G Q Q S S K S D R V V A S N V K V E T Q S D E E N G R A C E M N G E E C A E D L R M L D A S G E K M N G S H R D Q G S S A L S G V G G I R L P N G K L K C D I C G I I C I G P N V L M V H K R S H T G E R P F Q C N Q C G A S F T Q K G N L L R H I K L H S G E K P F K C H L C N Y A C R R R D A L T G H L R T H S V G K P H K C G Y C G R S Y K Q R S S L E E H K E R C H N Y L E S M G L P G T L Y P V I K E E T N H S E M A E D L C K I G S E R S L V L D R L A S N V A K R K S S M P Q K F L G D K G L S D T P Y D S S A S Y E K E N E M M K S H V M D Q A I N N A I N Y L G A E S L R P L V Q T P P G G S E V V P V I S P M Y Q L H K P L A E G T P R S N H S A Q D S A V E N L L L L S K A K L V P S E R E A S P S N S C Q D S T D T E S N N E E Q R S G L I Y L T N H I A P H A R N G L S L K E E H R A Y D L L R A A S E N S Q D A L R V V S T S G E Q M K V Y K C E H C R V L F L D H V M Y T I H M G C H G F R D P F E C N M C G Y H S Q D R Y E F S S H I T R G E H R F H M S
Full Length
60.6 kDa
1 to 519
Recombinant
His tag N-Terminus, T7 tag N-Terminus
Liquid
ab169877 was expressed in E. coli as inclusion bodies, refolded and chromatographically purified.
Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
Belongs to the Ikaros C2H2-type zinc-finger protein family.
Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
Nucleus
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.
Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.
The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.
Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.
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