Recombinant human IKK beta protein (Active) is a Human Full Length protein, in the 1 to 662 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M S W S P S L T T Q T C G A W E M K E R L G T G G F G N V I R W H N Q E T G E Q I A I K Q C R Q E L S P R N R E R W C L E I Q I M R R L T H P N V V A A R D V P E G M Q N L A P N D L P L L A M E Y C Q G G D L R K Y L N Q F E N C C G L R E G A I L T L L S D I A S A L R Y L H E N R I I H R D L K P E N I V L Q Q G E Q R L I H K I I D L G Y A K E L D Q G S L C T S F V G T L Q Y L A P E L L E Q Q K Y T V T V D Y W S F G T L A F E C I T G F R P F L P N W Q P V Q W H S K V R Q K S E V D I V V S E D L N G T V K F S S S L P Y P N N L N S V L A E R L E K W L Q L M L M W H P R Q R G T D P T Y G P N G C F K A L D D I L N L K L V H I L N M V T G T I H T Y P V T E D E S L Q S L K A R I Q Q D T G I P E E D Q E L L Q E A G L A L I P D K P A T Q C I S D G K L N E G H T L D M D L V F L F D N S K I T Y E T Q I S P R P Q P E S V S C I L Q E P K R N L A F F Q L R K V W G Q V W H S I Q T L K E D C N R L Q Q G Q R A A M M N L L R N N S C L S K M K N S M A S M S Q Q L K A K L D F F K T S I Q I D L E K Y S E Q T E F G I T S D K L L L A W R E M E Q A V E L C G R E N E V K L L V E R M M A L Q T D I V D L Q R S P M G R K Q G G T L D D L E E Q A R E L Y R R L R E K P R D Q R T E G D S Q E M V R L L L Q A I Q S F E K K V R V I Y T Q L S K T V V C K Q K A L E L L P K V E E V V S L M N E D E K T V V R L Q E K R Q K E L W N L L K I A C S K V R G P V S G S P D S M N A S R L S Q P G Q L M S Q P S T A S N S L P E P A K K S E E L V A E A H N L C T L L E N A I Q D T V R E Q D Q S F T A L D W S W L Q T E E E E H S C L E Q A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:30337470, PubMed:9346484). Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation (PubMed:9346484). Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE (PubMed:11297557, PubMed:14673179, PubMed:20410276, PubMed:21138416). IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs (PubMed:11297557, PubMed:20410276, PubMed:21138416). Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor (PubMed:15084260). Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 (PubMed:17213322, PubMed:19716809). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (PubMed:25326418). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at 'Thr-749' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses (PubMed:38621137). IKBKB-mediated phosphorylation of STAT1 at 'Thr-749' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription (PubMed:32209697).
Alternative names
IKKB, IKBKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, Serine/threonine protein kinase IKBKB, IKK-2, IKK2, NFKBIKB
Recommended products
Recombinant human IKK beta protein (Active) is a Human Full Length protein, in the 1 to 662 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of IKK beta was determined to be 58 nmol/min/mg.
- Accession
- O14920-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris-HCl buffer, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M S W S P S L T T Q T C G A W E M K E R L G T G G F G N V I R W H N Q E T G E Q I A I K Q C R Q E L S P R N R E R W C L E I Q I M R R L T H P N V V A A R D V P E G M Q N L A P N D L P L L A M E Y C Q G G D L R K Y L N Q F E N C C G L R E G A I L T L L S D I A S A L R Y L H E N R I I H R D L K P E N I V L Q Q G E Q R L I H K I I D L G Y A K E L D Q G S L C T S F V G T L Q Y L A P E L L E Q Q K Y T V T V D Y W S F G T L A F E C I T G F R P F L P N W Q P V Q W H S K V R Q K S E V D I V V S E D L N G T V K F S S S L P Y P N N L N S V L A E R L E K W L Q L M L M W H P R Q R G T D P T Y G P N G C F K A L D D I L N L K L V H I L N M V T G T I H T Y P V T E D E S L Q S L K A R I Q Q D T G I P E E D Q E L L Q E A G L A L I P D K P A T Q C I S D G K L N E G H T L D M D L V F L F D N S K I T Y E T Q I S P R P Q P E S V S C I L Q E P K R N L A F F Q L R K V W G Q V W H S I Q T L K E D C N R L Q Q G Q R A A M M N L L R N N S C L S K M K N S M A S M S Q Q L K A K L D F F K T S I Q I D L E K Y S E Q T E F G I T S D K L L L A W R E M E Q A V E L C G R E N E V K L L V E R M M A L Q T D I V D L Q R S P M G R K Q G G T L D D L E E Q A R E L Y R R L R E K P R D Q R T E G D S Q E M V R L L L Q A I Q S F E K K V R V I Y T Q L S K T V V C K Q K A L E L L P K V E E V V S L M N E D E K T V V R L Q E K R Q K E L W N L L K I A C S K V R G P V S G S P D S M N A S R L S Q P G Q L M S Q P S T A S N S L P E P A K K S E E L V A E A H N L C T L L E N A I Q D T V R E Q D Q S F T A L D W S W L Q T E E E E H S C L E Q A S
- Accession
- O14920
- Protein length
- Full Length
- Predicted molecular weight
- 105 kDa
- Amino acids
- 1 to 662
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity assessed by densitometry. Affinity purified.
General info
- Function
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:30337470, PubMed:9346484). Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation (PubMed:9346484). Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE (PubMed:11297557, PubMed:14673179, PubMed:20410276, PubMed:21138416). IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs (PubMed:11297557, PubMed:20410276, PubMed:21138416). Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor (PubMed:15084260). Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 (PubMed:17213322, PubMed:19716809). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (PubMed:25326418). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at 'Thr-749' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses (PubMed:38621137). IKBKB-mediated phosphorylation of STAT1 at 'Thr-749' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription (PubMed:32209697).
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
- Post-translational modifications
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity (PubMed:10022904, PubMed:16207722). Phosphorylated by MAP3K7/TAK1 in response to NOD1 and NOD2 signaling, promoting activation and phosphorylation of NF-kappa-B inhibitors, leading to NF-kappa-B activation (PubMed:11460167). Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response (PubMed:10195894). Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription (PubMed:10783893). Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B (PubMed:18930133).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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4 product images
Functional Studies - Recombinant human IKK beta protein (Active) (ab177584)
The specific activity of IKK beta (ab177584) was determined to be 49 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human IKK beta protein (Active) (ab177584)
SDS PAGE analysis of ab177584
SDS-PAGE - Recombinant human IKK beta protein (Active) (ab177584)
The purity of ab177584 was determined to be >95% by densitometry.
Functional Studies - Recombinant human IKK beta protein (Active) (ab177584)
The specific activity of IKK beta was determined to be 58 nmol/min/mg.
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