Recombinant human IKKi/IKKe protein is a Human Full Length protein, in the 1 to 716 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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- Journal of molecular cell biology 11:158-1692018Host HDAC4 regulates the antiviral response by inhibiting the phosphorylation of IRF3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Yang,Jielin Tang,Rongjuan Pei,XiaoXiao Gao,Jing Guo,Chonghui Xu,Yun Wang,Qian Wang,Chunchen Wu,Yuan Zhou,Xue Hu,He Zhao,Yanyi Wang,Xinwen Chen,Jizheng ChenPubMed 29800227
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M Q S T A N Y L W H T D D L L G Q G A T A S V Y K A R N K K S G E L V A V K V F N T T S Y L R P R E V Q V R E F E V L R K L N H Q N I V K L F A V E E T G G S R Q K V L V M E Y C S S G S L L S V L E S P E N A F G L P E D E F L V V L R C V V A G M N H L R E N G I V H R D I K P G N I M R L V G E E G Q S I Y K L T D F G A A R E L D D D E K F V S V Y G T E E Y L H P D M Y E R A V L R K P Q Q K A F G V T V D L W S I G V T L Y H A A T G S L P F I P F G G P R R N K E I M Y R I T T E K P A G A I A G A Q R R E N G P L E W S Y T L P I T C Q L S L G L Q S Q L V P I L A N I L E V E Q A K C W G F D Q F F A E T S D I L Q R V V V H V F S L S Q A V L H H I Y I H A H N T I A I F Q E A V H K Q T S V A P R H Q E Y L F E G H L C V L E P S V S A Q H I A H T T A S S P L T L F S T A I P K G L A F R D P A L D V P K F V P K V D L Q A D Y N T A K G V L G A G Y Q A L R L A R A L L D G Q E L M F R G L H W V M E V L Q A T C R R T L E V A R T S L L Y L S S S L G T E R F S S V A G T P E I Q E L K A A A E L R S R L R T L A E V L S R C S Q N I T E T Q E S L S S L N R E L V K S R D Q V H E D R S I Q Q I Q C C L D K M N F I Y K Q F K K S R M R P G L G Y N E E Q I H K L D K V N F S H L A K R L L Q V F Q E E C V Q K Y Q A S L V T H G K R M R V V H E T R N H L R L V G C S V A A C N T E A Q G V Q E S L S K L L E E L S H Q L L Q D R A K G A Q A S P P P I A P Y P S P T R K D L L L H M Q E L C E G M K L L A S D L L D N N R I I E R L N R V P A P P D V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.
Alternative names
IKKE, IKKI, KIAA0151, IKBKE, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, Inducible I kappa-B kinase, IKK-i
Recommended products
Recombinant human IKKi/IKKe protein is a Human Full Length protein, in the 1 to 716 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab201367 was determined to be 12 nmol/min/mg as determined by kinase assay.
- Accession
- Q14164-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M Q S T A N Y L W H T D D L L G Q G A T A S V Y K A R N K K S G E L V A V K V F N T T S Y L R P R E V Q V R E F E V L R K L N H Q N I V K L F A V E E T G G S R Q K V L V M E Y C S S G S L L S V L E S P E N A F G L P E D E F L V V L R C V V A G M N H L R E N G I V H R D I K P G N I M R L V G E E G Q S I Y K L T D F G A A R E L D D D E K F V S V Y G T E E Y L H P D M Y E R A V L R K P Q Q K A F G V T V D L W S I G V T L Y H A A T G S L P F I P F G G P R R N K E I M Y R I T T E K P A G A I A G A Q R R E N G P L E W S Y T L P I T C Q L S L G L Q S Q L V P I L A N I L E V E Q A K C W G F D Q F F A E T S D I L Q R V V V H V F S L S Q A V L H H I Y I H A H N T I A I F Q E A V H K Q T S V A P R H Q E Y L F E G H L C V L E P S V S A Q H I A H T T A S S P L T L F S T A I P K G L A F R D P A L D V P K F V P K V D L Q A D Y N T A K G V L G A G Y Q A L R L A R A L L D G Q E L M F R G L H W V M E V L Q A T C R R T L E V A R T S L L Y L S S S L G T E R F S S V A G T P E I Q E L K A A A E L R S R L R T L A E V L S R C S Q N I T E T Q E S L S S L N R E L V K S R D Q V H E D R S I Q Q I Q C C L D K M N F I Y K Q F K K S R M R P G L G Y N E E Q I H K L D K V N F S H L A K R L L Q V F Q E E C V Q K Y Q A S L V T H G K R M R V V H E T R N H L R L V G C S V A A C N T E A Q G V Q E S L S K L L E E L S H Q L L Q D R A K G A Q A S P P P I A P Y P S P T R K D L L L H M Q E L C E G M K L L A S D L L D N N R I I E R L N R V P A P P D V
- Accession
- Q14164
- Protein length
- Full Length
- Predicted molecular weight
- 110 kDa
- Amino acids
- 1 to 716
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
- Post-translational modifications
Autophosphorylated and phosphorylated by IKBKB/IKKB. Phosphorylation at Ser-172 is enhanced by the interaction with DDX3X. Phosphorylated at Thr-501 upon IFN activation.
- Subcellular localisation
- Nucleus, PML body
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
IKB alpha peptide ab204863 (IKB alpha peptide) can be utilized as a substrate for assessing kinase activity
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4 product images
Functional Studies - Recombinant human IKKi/IKKe protein (ab201367)
The specific activity of IKKi/IKKe (ab201367) was determined to be 11 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human IKKi/IKKe protein (ab201367)
SDS PAGE analysis of ab201367
Functional Studies - Recombinant human IKKi/IKKe protein (ab201367)
Specific activity of ab201367
SDS-PAGE - Recombinant human IKKi/IKKe protein (ab201367)
SDS PAGE analysis of ab201367
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