Skip to main content

Recombinant human IL-1 beta protein (Active) is a Human Full Length protein, in the 117 to 269 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

Purity

>95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biologically active

Yes

Sequence

A P V R S L N C T L R D S Q Q K S L V M S G P Y E L K A L H L Q G Q D M E Q Q V V F S M S F V Q G E E S N D K I P V A L

G L K E K N L Y L S C V L K D D K P T L Q L E S V D P K N Y P K K K M E K R F V F N K I E I N N K L E F E S A Q F P N W

Y I S T S Q A E N M P V F L G G T K G G Q D I T D F T M Q F V S S

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Recombinant human IL-1 beta protein (Active) is a Human Full Length protein, in the 117 to 269 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biological activity

Fully biologically active when compared to standard. ED50 is ≤ 0.7399 ng /ml, corresponding to a specific activity of 1.35 x 106 units/mg.

Predicted molecular weight

17.43 kDa

Amino acids

117 to 269

Sequence

A P V R S L N C T L R D S Q Q K S L V M S G P Y E L K A L H L Q G Q D M E Q Q V V F S M S F V Q G E E S N D K I P V A L

G L K E K N L Y L S C V L K D D K P T L Q L E S V D P K N Y P K K K M E K R F V F N K I E I N N K L E F E S A Q F P N W

Y I S T S Q A E N M P V F L G G T K G G Q D I T D F T M Q F V S S

Accession
P01584-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute in PBS, aliquot and store at -80 C for 12 months or 4 C for 1 week. Avoid repeated freeze thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
Loading...
Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

Purity by HPLC >=95%.

General info

Function

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819).

Sequence similarities

Belongs to the IL-1 family.

Post-translational modifications

Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Subcellular localisation

Lysosome

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com