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Recombinant human IL-1 beta protein (Active) is a Human Full Length protein, in the 117 to 269 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Sequence
A P V R S L N C T L R D S Q Q K S L V M S G P Y E L K A L H L Q G Q D M E Q Q V V F S M S F V Q G E E S N D K I P V A L
G L K E K N L Y L S C V L K D D K P T L Q L E S V D P K N Y P K K K M E K R F V F N K I E I N N K L E F E S A Q F P N W
Y I S T S Q A E N M P V F L G G T K G G Q D I T D F T M Q F V S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human IL-1 beta protein (Active) is a Human Full Length protein, in the 117 to 269 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Biological activity
Fully biologically active when compared to standard. ED50 is ≤ 0.7399 ng /ml, corresponding to a specific activity of 1.35 x 106 units/mg.
- Predicted molecular weight
17.43 kDa
- Amino acids
117 to 269
- Sequence
A P V R S L N C T L R D S Q Q K S L V M S G P Y E L K A L H L Q G Q D M E Q Q V V F S M S F V Q G E E S N D K I P V A L
G L K E K N L Y L S C V L K D D K P T L Q L E S V D P K N Y P K K K M E K R F V F N K I E I N N K L E F E S A Q F P N W
Y I S T S Q A E N M P V F L G G T K G G Q D I T D F T M Q F V S S- Accession
- P01584-1
- Protein length
Full Length
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute in PBS, aliquot and store at -80 C for 12 months or 4 C for 1 week. Avoid repeated freeze thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819).
- Sequence similarities
Belongs to the IL-1 family.
- Post-translational modifications
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
- Subcellular localisation
Lysosome
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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