Recombinant human IL-1 beta protein (Active) is a Human Full Length protein, in the 117 to 269 aa range with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, Functional studies, Cell Culture and more. The predicted molecular weight of ab259387 protein is 17.4 kDa.
- Save time and ensure accurate results - use our recombinant IL-1 beta protein as a control
- Tag-free, ensuring its native conformation
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
A P V R S L N C T L R D S Q Q K S L V M S G P Y E L K A L H L Q G Q D M E Q Q V V F S M S F V Q G E E S N D K I P V A L G L K E K N L Y L S C V L K D D K P T L Q L E S V D P K N Y P K K K M E K R F V F N K I E I N N K L E F E S A Q F P N W Y I S T S Q A E N M P V F L G G T K G G Q D I T D F T M Q F V S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).
Alternative names
IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin
Recommended products
Recombinant human IL-1 beta protein (Active) is a Human Full Length protein, in the 117 to 269 aa range with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, Functional studies, Cell Culture and more. The predicted molecular weight of ab259387 protein is 17.4 kDa.
- Save time and ensure accurate results - use our recombinant IL-1 beta protein as a control
- Tag-free, ensuring its native conformation
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active when compared to standard. ED50 is ≤ 0.7399 ng /ml, corresponding to a specific activity of 1.35 x 106 units/mg.
- Accession
- P01584-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A P V R S L N C T L R D S Q Q K S L V M S G P Y E L K A L H L Q G Q D M E Q Q V V F S M S F V Q G E E S N D K I P V A L G L K E K N L Y L S C V L K D D K P T L Q L E S V D P K N Y P K K K M E K R F V F N K I E I N N K L E F E S A Q F P N W Y I S T S Q A E N M P V F L G G T K G G Q D I T D F T M Q F V S S
- Accession
- P01584
- Protein length
- Full Length
- Predicted molecular weight
- 17.43 kDa
- Amino acids
- 117 to 269
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).
- Sequence similarities
Belongs to the IL-1 family.
- Post-translational modifications
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our recombinant human IL-1 beta protein ab259387 as a control.
The ab259387 IL-1 beta protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC. Analyze your IL-1 beta ELISA data using the ab259387 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE here
Check out our ELISA protocol for more information here.
Premium Bioactive Protein range
The ab259387 IL-1 beta protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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6 product images
Functional Studies - Recombinant human IL-1 beta protein (Active) (ab259387)
Fully biologically active when compared to standard. ED50 is ≤ 0.7399 ng /ml, corresponding to a specific activity of 1.35 x 106 units/mg.
SDS-PAGE - Recombinant human IL-1 beta protein (Active) (ab259387)
SDS-PAGE analysis of ab259387.
HPLC - Recombinant human IL-1 beta protein (Active) (ab259387)
Purity: 100%
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Mass Spectrometry - Recombinant human IL-1 beta protein (Active) (ab259387)
M + 1.02 Da (calc mass 17433.98)
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
Sandwich ELISA - Recombinant human IL-1 beta protein (Active) (ab259387)
Background subtracted standard curve using Human IL-1 beta Antibody Pair - BSA and Azide free (Human/Monkey IL-1beta Antibody Pair - BSA and Azide free ab241807) and Recombinant human IL-1 beta protein (Active) (ab259387) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
Sandwich ELISA - Recombinant human IL-1 beta protein (Active) (ab259387)
Human IL-6 concentration was interpolated from the IL-6 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-6 ELISA kit (Human IL-6 ELISA Kit ab178013). Wild-type and IL-6 knockout A549 cells (Human IL-6 knockout A549 cell line ab273751) were assessed in duplicate (n=2). Cells were either treated with 20 ng/mL active recombinant human IL-1 beta protein (ab259387) for 24 h to induce expression of IL-6 or not treated with IL-1 beta. Data are represented as the mean and error bars represent standard deviation.
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