Recombinant human IL-10 protein (Active) is a Human Fragment protein, in the 26 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Images
Publications
Filter by
- iScience 25:1051472022Vascular restenosis reduction with platelet membrane coated nanoparticle directed M2 macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengshi Li,Zhihua Rong,Rui Zhang,Shuai Niu,Xiao Di,Leng Ni,Changwei LiuPubMed 36274932
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
Alternative names
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Recommended products
Recombinant human IL-10 protein (Active) is a Human Fragment protein, in the 26 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.
ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.
- Accession
- P22301-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
- Accession
- P22301
- Protein length
- Fragment
- Predicted molecular weight
- 17.99 kDa
- Amino acids
- 26 to 178
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
- Sequence similarities
Belongs to the IL-10 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant human IL-10 protein (Active) (ab259402)
Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.
ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.
SDS-PAGE - Recombinant human IL-10 protein (Active) (ab259402)
SDS-PAGE analysis of ab259402.
HPLC - Recombinant human IL-10 protein (Active) (ab259402)
Purity = 97%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50°C.
Mass Spectrometry - Recombinant human IL-10 protein (Active) (ab259402)
M+0.17 Da (Calc. mass 17991.83 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60°C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com