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Recombinant human IL-10 protein (Active) is a Human Fragment protein, in the 26 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Sequence
S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human IL-10 protein (Active) is a Human Fragment protein, in the 26 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Biological activity
Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.
ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.
- Predicted molecular weight
17.99 kDa
- Amino acids
26 to 178
- Sequence
S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
- Accession
- P22301-1
- Protein length
Fragment
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
- Sequence similarities
Belongs to the IL-10 family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product will no longer be available once inventory is depleted. It is being replaced by ab284660. This protein has an improved amino acid sequence, 19-178, matching the mature full length IL-10 protein.
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.
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