Recombinant human IL-10 protein (Active) is a Human Fragment protein, in the 26 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
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- iScience 25:1051472022Vascular restenosis reduction with platelet membrane coated nanoparticle directed M2 macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengshi Li,Zhihua Rong,Rui Zhang,Shuai Niu,Xiao Di,Leng Ni,Changwei LiuPubMed 36274932
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
Amino acid sequence
S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
Alternative names
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Recommended products
Recombinant human IL-10 protein (Active) is a Human Fragment protein, in the 26 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Biological activity
Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.
ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.
- Accession
- P22301-1
- Animal free
Yes
- Species
Human
- Reconstitution
Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
- Accession
- Protein length
Fragment
- Predicted molecular weight
17.99 kDa
- Amino acids
26 to 178
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
- Sequence similarities
Belongs to the IL-10 family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product will no longer be available once inventory is depleted. It is being replaced by Recombinant human IL-10 protein (Active) ab284660. This protein has an improved amino acid sequence, 19-178, matching the mature full length IL-10 protein.
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.
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Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant human IL-10 protein (Active) (ab259402)
Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.
ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.
SDS-PAGE - Recombinant human IL-10 protein (Active) (ab259402)
SDS-PAGE analysis of ab259402.
HPLC - Recombinant human IL-10 protein (Active) (ab259402)
Purity = 97%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50°C.
Mass Spectrometry - Recombinant human IL-10 protein (Active) (ab259402)
M+0.17 Da (Calc. mass 17991.83 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60°C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
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