Recombinant human IL-10 protein (Active) is a Human Full Length protein, in the 19 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Biological Activity.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Biological Activity
- Biologically active
- Yes
Amino acid sequence
S P G Q G T Q S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Biological Activity | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
Alternative names
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Recommended products
Recombinant human IL-10 protein (Active) is a Human Full Length protein, in the 19 to 178 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Biological Activity.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Biological Activity
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by dose dependent proliferation of MC/9 cells. ED50 is ≤2.759 ng/mL, corresponding to a specific activity of 3.63 x 105 units/mg
- Accession
- P22301-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- S P G Q G T Q S E N S C T H F P G N L P N M L R D L R D A F S R V K T F F Q M K D Q L D N L L L K E S L L E D F K G Y L G C Q A L S E M I Q F Y L E E V M P Q A E N Q D P D I K A H V N S L G E N L K T L R L R L R R C H R F L P C E N K S K A V E Q V K N A F N K L Q E K G I Y K A M S E F D I F I N Y I E A Y M T M K I R N
- Accession
- P22301
- Protein length
- Full Length
- Actual molecular weight
- 18.6 kDa
- Amino acids
- 19 to 178
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
- Sequence similarities
Belongs to the IL-10 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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4 product images
Biological Activity - Recombinant human IL-10 protein (Active) (ab284660)
Fully biologically active determined by dose dependent proliferation of MC/9 cells. ED50 is ≤2.759 ng/mL, corresponding to a specific activity of 3.63 x 105 units/mg
SDS-PAGE - Recombinant human IL-10 protein (Active) (ab284660)
SDS-page analysis of ab284660
Mass Spectrometry - Recombinant human IL-10 protein (Active) (ab284660)
Mass determination by ESI-TOF.
Predicted MW is 18647.43 Da (+/- 10 Da by ESI-TOF). Observed MW is 18648.85 Da.
HPLC - Recombinant human IL-10 protein (Active) (ab284660)
HPLC analysis of ab284660
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Product protocols
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