Recombinant Human IL-10RA protein (His tag) is a Human Fragment protein, in the 22 to 235 aa range, expressed in Insect, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Insect cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
H G T E L P S P P S V W F E A E F F H H I L H W T P I P N Q S E S T C Y E V A L L R Y G I E S W N S I S N C S Q T L S Y D L T A V T L D L Y H S N G Y R A R V R A V D G S R H S N W T V T N T R F S V D E V T L T V G S V N L E I H N G F I L G K I Q L P R P K M A P A N D T Y E S I F S H F R E Y E I A I R K V P G N F T F T H K K V K H E N F S L L T S G E V G E F C V Q V K P S V A S R S N K G M W S K E E C I S L T R Q Y F T V T N H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes 28-40kDa (SDS–PAGE under reducing conditions) |
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Target data
Function
Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683).
Alternative names
CD210, IL10R, IL10RA, Interleukin-10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1
Recommended products
Recombinant Human IL-10RA protein (His tag) is a Human Fragment protein, in the 22 to 235 aa range, expressed in Insect, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Insect cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q13651-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- H G T E L P S P P S V W F E A E F F H H I L H W T P I P N Q S E S T C Y E V A L L R Y G I E S W N S I S N C S Q T L S Y D L T A V T L D L Y H S N G Y R A R V R A V D G S R H S N W T V T N T R F S V D E V T L T V G S V N L E I H N G F I L G K I Q L P R P K M A P A N D T Y E S I F S H F R E Y E I A I R K V P G N F T F T H K K V K H E N F S L L T S G E V G E F C V Q V K P S V A S R S N K G M W S K E E C I S L T R Q Y F T V T N H H H H H H
- Accession
- Q13651
- Protein length
- Fragment
- Predicted molecular weight
- 25.2 kDa
- Amino acids
- 22 to 235
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683).
- Sequence similarities
Belongs to the type II cytokine receptor family.
- Post-translational modifications
Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human IL-10RA protein (His tag) (ab201890)
15% SDS-PAGE analysis of ab201890 (3 μg)
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