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AB281801

Recombinant human IL-11 protein (Active)

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Recombinant human IL-11 protein (Active) is a Human Full Length protein, in the 22 to 199 aa range, expressed in HEK 293 cells, with >95%, < 0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11

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Mass Spectrometry - Recombinant human IL-11 protein (Active) (AB281801)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human IL-11 protein (Active) (AB281801)

Mass determination by ESI-TOF.

Predicted MW is 19201.44 Da. (+/- 10 Da by ESI-TOF). Observed MW is 19202.46 Da. Additional masses 19048.37 are due to residual O-glycans.

Functional Studies - Recombinant human IL-11 protein (Active) (AB281801)
  • FuncS

Supplier Data

Functional Studies - Recombinant human IL-11 protein (Active) (AB281801)

Fully biologically active as determined by the dose dependent proliferation of TF-1 cells. ED50 for this effect is ≤4.69 ng/ml corresponding to a specific activity of 2.13 x 105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3385910-1

SDS-PAGE - Recombinant human IL-11 protein (Active) (AB281801)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IL-11 protein (Active) (AB281801)

SDS-PAGE analysis of ab281801.

HPLC - Recombinant human IL-11 protein (Active) (AB281801)
  • HPLC

Supplier Data

HPLC - Recombinant human IL-11 protein (Active) (AB281801)

HPLC analysis of ab281801.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Cell Culture, SDS-PAGE, Mass Spec, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active as determined by the dose dependent proliferation of TF-1 cells. ED50 for this effect is ≤4.69 ng/ml corresponding to a specific activity of 2.13 x 105 units/mg.

Accession

P20809

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"PGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL","proteinLength":"Full Length","predictedMolecularWeight":"19 kDa","actualMolecularWeight":"19.2 kDa","aminoAcidEnd":199,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P20809","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

>95% by HPLC

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General info

Function

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed : 12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed : 30279168).

Sequence similarities

Belongs to the IL-6 superfamily.

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Product protocols

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Target data

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed : 12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed : 30279168).
See full target information IL11
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