Recombinant human IL-11 protein (Active) is a Human Full Length protein, in the 22 to 199 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
P G P P P G P P R V S P D P R A E L D S T V L L T R S L L A D T R Q L A A Q L R D K F P A D G D H N L D S L P T L A M S A G A L G A L Q L P G V L T R L R A D L L S Y L R H V Q W L R R A G G S S L K T L E P E L G T L Q A R L D R L L R R L Q L L M S R L A L P Q P P P D P P A P P L A P P S S A W G G I R A A H A I L G G L H L T L D W A V R G L L L L K T R L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed:12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed:30279168).
Alternative names
Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11
Recommended products
Recombinant human IL-11 protein (Active) is a Human Full Length protein, in the 22 to 199 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active as determined by the dose dependent proliferation of TF-1 cells. ED50 for this effect is ≤4.69 ng/ml corresponding to a specific activity of 2.13 x 105 units/mg.
- Accession
- P20809-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- P G P P P G P P R V S P D P R A E L D S T V L L T R S L L A D T R Q L A A Q L R D K F P A D G D H N L D S L P T L A M S A G A L G A L Q L P G V L T R L R A D L L S Y L R H V Q W L R R A G G S S L K T L E P E L G T L Q A R L D R L L R R L Q L L M S R L A L P Q P P P D P P A P P L A P P S S A W G G I R A A H A I L G G L H L T L D W A V R G L L L L K T R L
- Accession
- P20809
- Protein length
- Full Length
- Predicted molecular weight
- 19 kDa
- Actual molecular weight
- 19.2 kDa
- Amino acids
- 22 to 199
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>95% by HPLC
General info
- Function
Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed:12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed:30279168).
- Sequence similarities
Belongs to the IL-6 superfamily.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant human IL-11 protein (Active) (ab281801)
Fully biologically active as determined by the dose dependent proliferation of TF-1 cells. ED50 for this effect is ≤4.69 ng/ml corresponding to a specific activity of 2.13 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3385910-1
SDS-PAGE - Recombinant human IL-11 protein (Active) (ab281801)
SDS-PAGE analysis of ab281801.
Mass Spectrometry - Recombinant human IL-11 protein (Active) (ab281801)
Mass determination by ESI-TOF.
Predicted MW is 19201.44 Da. (+/- 10 Da by ESI-TOF). Observed MW is 19202.46 Da. Additional masses 19048.37 are due to residual O-glycans.
HPLC - Recombinant human IL-11 protein (Active) (ab281801)
HPLC analysis of ab281801.
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Product protocols
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