Recombinant Human IL-12 p40 protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
I W E L K K D V Y V V E L D W Y P D A P G E M V V L T C D T P E E D G I T W T L D Q S S E V L G S G K T L T I Q V K E F G D A G Q Y T C H K G G E V L S H S L L L L H K K E D G I W S T D I L K D Q K E P K N K T F L R C E A K N Y S G R F T C W W L T T I S T D L T F S V K S S R G S S D P Q G V T C G A A T L S A E R V R G D N K E Y E Y S V E C Q E D S A C P A A E E S L P I E V M V D A V H K L K Y E N Y T S S F F I R D I I K P D P P K N L Q L K P L K N S R Q V E V S W E Y P D T W S T P H S Y F S L T F C V Q V Q G K S K R E K K D R V F T D K T S A T V I C R K N A S I S V R A Q D R Y Y S S S W S E W A S V P C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Alternative names
NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40
Recommended products
Recombinant Human IL-12 p40 protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P29460-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- I W E L K K D V Y V V E L D W Y P D A P G E M V V L T C D T P E E D G I T W T L D Q S S E V L G S G K T L T I Q V K E F G D A G Q Y T C H K G G E V L S H S L L L L H K K E D G I W S T D I L K D Q K E P K N K T F L R C E A K N Y S G R F T C W W L T T I S T D L T F S V K S S R G S S D P Q G V T C G A A T L S A E R V R G D N K E Y E Y S V E C Q E D S A C P A A E E S L P I E V M V D A V H K L K Y E N Y T S S F F I R D I I K P D P P K N L Q L K P L K N S R Q V E V S W E Y P D T W S T P H S Y F S L T F C V Q V Q G K S K R E K K D R V F T D K T S A T V I C R K N A S I S V R A Q D R Y Y S S S W S E W A S V P C S
- Accession
- P29460
- Protein length
- Full Length
- Predicted molecular weight
- 36.6 kDa
- Actual molecular weight
- 36.6 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
- Sequence similarities
Belongs to the IL-12B family.
- Post-translational modifications
Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Product promise
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Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human IL-12 p40 protein (His-tag) (ab322315)
SDS-PAGE analysis of ab322315 under reducing conditions for 2ug protein.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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