Recombinant human IL-12 p70 protein (Active)

• Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human IL-12 p70 protein (Active) (AB259418)

M + 2.97 Da (Calc mass 34754.03 Da).

The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water : FA (99.9 : 0.1 v/v) and 15 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) to 55 % water : FA (99.9 : 0.1 v/v) and 45 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

• Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human IL-12 p70 protein (Active) (AB259418)

M + 2.8 Da (Calc. mass 22599.2 Da).

The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water : FA (99.9 : 0.1 v/v) and 15 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) to 55 % water : FA (99.9 : 0.1 v/v) and 45 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

• Biological Activity

Lab

Biological Activity - Recombinant human IL-12 p70 protein (Active) (AB259418)

Fully biologically active in a dose-dependent proliferation of NK-92 cell line. ED50 is ≤ 2.35 ng/ml, corresponding to a specific activity of 0.42 x 10⁶ units/mg.

• SDS-PAGE

Lab

SDS-PAGE - Recombinant human IL-12 p70 protein (Active) (AB259418)

SDS-PAGE analysis of ab259418.

Protein is a heterodimer.

• HPLC

Supplier Data

HPLC - Recombinant human IL-12 p70 protein (Active) (AB259418)

Purity 96%.

The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water : TFA (99.9 : 0.1 v/v) and 20 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) to 20 % water : TFA (99.9 : 0.1 v/v) and 80 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.