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Recombinant Human IL-13 protein (Active) is a Human Full Length protein, in the 25 to 146 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Alternative names=Interleukin-13, IL-13, IL13, NC30
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
- Actual molecular weight
13.35 kDa
- Sequence
L T C L G G F A S P G P V P P S T A L R E L I E E L V N I T Q N Q K A P L C N G S M V W S I N L T A G M Y C A A L E S L I N V S G C S A I E K T Q R M L S G F C P H K V S A G Q F S S L H V R D T K I E V A Q F V K D L L L H L K K L F R E G R F N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human IL-13 protein (Active) is a Human Full Length protein, in the 25 to 146 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Alternative names=Interleukin-13, IL-13, IL13, NC30
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
- Biological activity
Fully biologically active determined bydose dependent proliferation of TF-1 cells.
ED50 is ≤ 18.99 ng/ml, corresponding to a specific activity of 5.3 x 104 units/mg.
- Predicted molecular weight
13.35 kDa
- Actual molecular weight
13.35 kDa
- Amino acids
25 to 146
- Sequence
L T C L G G F A S P G P V P P S T A L R E L I E E L V N I T Q N Q K A P L C N G S M V W S I N L T A G M Y C A A L E S L I N V S G C S A I E K T Q R M L S G F C P H K V S A G Q F S S L H V R D T K I E V A Q F V K D L L L H L K K L F R E G R F N
- Accession
- P35225-1
- Protein length
Full Length
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
- Additional notes
=95% by HPLC
General info
- Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages (By similarity).
- Sequence similarities
Belongs to the IL-4/IL-13 family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
For research use only and are not intended for diagnostic or therapeutic use, not for use in humans.
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