Recombinant human IL-15 protein (Active) is a Human Full Length protein, in the 49 to 162 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Images
Publications
Filter by
- eLife 10:2021Evolutionary transcriptomics implicates in the origins of implantation and regulation of gestation length.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMirna Marinić,Katelyn Mika,Sravanthi Chigurupati,Vincent J LynchPubMed 33522483
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
N W V N V I S D L K K I E D L I Q S M H I D A T L Y T E S D V H P S C K V T A M K C F L L E L Q V I S L E S G D A S I H D T V E N L I I L A N N S L S S N G N V T E S G C K E C E E L E E K N I K E F L Q S F V H I V Q M F I N T S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed:15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed:8178155, PubMed:9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed:9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed:10233906, PubMed:23104097, PubMed:8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed:7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
Alternative names
Interleukin-15, IL-15, IL15
Recommended products
Recombinant human IL-15 protein (Active) is a Human Full Length protein, in the 49 to 162 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- sELISA, SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent proliferation of KHYG-1 cells.
ED50 is ≤1.28 ng/mL, corresponding to a specific activity of 7.8 x 105 units/mg.
- Accession
- P40933-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- N W V N V I S D L K K I E D L I Q S M H I D A T L Y T E S D V H P S C K V T A M K C F L L E L Q V I S L E S G D A S I H D T V E N L I I L A N N S L S S N G N V T E S G C K E C E E L E E K N I K E F L Q S F V H I V Q M F I N T S
- Accession
- P40933
- Protein length
- Full Length
- Predicted molecular weight
- 12.8 kDa
- Amino acids
- 49 to 162
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>= 95 % HPLC.
General info
- Function
Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed:15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed:8178155, PubMed:9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed:9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed:10233906, PubMed:23104097, PubMed:8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed:7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
- Sequence similarities
Belongs to the IL-15/IL-21 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Functional Studies - Recombinant human IL-15 protein (Active) (ab259403)
Functional analysis of ab259403.
Fully biologically active determined by the dose dependent proliferation of KHYG-1 cells.
ED50 is ≤1.28 ng/mL, corresponding to a specific activity of 7.8 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3379485-1
Mass Spectrometry - Recombinant human IL-15 protein (Active) (ab259403)
Mass determination by ESI-TOF.
Predicted MW is 12830.92 Da (+/- 10 Da by ESI-TOF). Observed MW is 12832.38 Da.
SDS-PAGE - Recombinant human IL-15 protein (Active) (ab259403)
SDS-PAGE analysis of ab259403.
HPLC - Recombinant human IL-15 protein (Active) (ab259403)
HPLC analysis of ab259403.
Sandwich ELISA - Recombinant human IL-15 protein (Active) (ab259403)
Background subtracted standard curve using Human IL-15 Antibody Pair - BSA and Azide free (Human IL-15 Antibody Pair - BSA and Azide free ab241883) and Recombinant human IL-15 protein (Active) (ab259403) in sandwich ELISA.
The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com