Recombinant human IL-15 protein (Active)
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(1 Publication)
Recombinant human IL-15 protein (Active) is a Human Full Length protein, in the 49 to 162 aa range, expressed in HEK 293 cells, with >95%, < 0.005 EU/µg endotoxin level, suitable for sELISA, SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.
View Alternative Names
Interleukin-15, IL-15, IL15
- FuncS
Supplier Data
Functional Studies - Recombinant human IL-15 protein (Active) (AB259403)
Functional analysis of ab259403.
Fully biologically active determined by the dose dependent proliferation of KHYG-1 cells.
ED50 is ≤1.28 ng/mL, corresponding to a specific activity of 7.8 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot : GR3379485-1
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant human IL-15 protein (Active) (AB259403)
Mass determination by ESI-TOF.
Predicted MW is 12830.92 Da (+/- 10 Da by ESI-TOF). Observed MW is 12832.38 Da.
- sELISA
Lab
Sandwich ELISA - Recombinant human IL-15 protein (Active) (AB259403)
Background subtracted standard curve using Human IL-15 Antibody Pair - BSA and Azide free (ab241883) and Recombinant human IL-15 protein (Active) (ab259403) in sandwich ELISA.
The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
- HPLC
Supplier Data
HPLC - Recombinant human IL-15 protein (Active) (AB259403)
HPLC analysis of ab259403.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human IL-15 protein (Active) (AB259403)
SDS-PAGE analysis of ab259403.
Complementary Products
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Specifications
Form
Lyophilized
Additional notes
>= 95 % HPLC.
General info
Function
Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8178155, PubMed : 9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed : 9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed : 10233906, PubMed : 23104097, PubMed : 8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed : 7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
Sequence similarities
Belongs to the IL-15/IL-21 family.
Subcellular localisation
Nucleus
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
eLife 10: PubMed33522483
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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