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Recombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for MS, SDS-PAGE, HPLC, Cell Culture.

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Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.1 EU/mg

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

MS, SDS-PAGE, HPLC, Cell Culture

Biologically active

Yes

Amino acid sequence

M Y F G K L E S K L S V I R N L N D Q V L F I D Q G N R P L F E D M T D S D C R D N A P R T I F I I S M Y K D S Q P R G M A V T I S V K C E K I S T L S C E N K I I S F K E M N P P D N I K D T K S D I I F F Q R S V P G H D N K M Q F E S S S Y E G Y F L A C E K E R D L F K L I L K K E D E L G D R S I M F T V Q N E D

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Reactivity data

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

Cell Culture

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed:14528293, PubMed:25500532). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable) (PubMed:10653850).

Alternative names

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Recombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for MS, SDS-PAGE, HPLC, Cell Culture.

Alternative names

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.1 EU/mg

Expression system

Escherichia coli

Applications

MS, SDS-PAGE, HPLC, Cell Culture

Biological activity

Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 107 units/mg.

Accession
Q14116-1
Animal free

Yes

Species

Human

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Concentration
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Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

M Y F G K L E S K L S V I R N L N D Q V L F I D Q G N R P L F E D M T D S D C R D N A P R T I F I I S M Y K D S Q P R G M A V T I S V K C E K I S T L S C E N K I I S F K E M N P P D N I K D T K S D I I F F Q R S V P G H D N K M Q F E S S S Y E G Y F L A C E K E R D L F K L I L K K E D E L G D R S I M F T V Q N E D

Accession

Q14116

Protein length

Full Length

Predicted molecular weight

19.4 kDa

Amino acids

37 to 193

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Lyophilized

Additional notes

SDS-PAGE >= 95%

General info

Function

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed:14528293, PubMed:25500532). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable) (PubMed:10653850).

Sequence similarities

Belongs to the IL-1 family.

Post-translational modifications

The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799), expandable thumbnail

    SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799)

    SDS-PAGE analysis of ab310799

  • Mass Spectrometry - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799), expandable thumbnail

    Mass Spectrometry - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799)

    Mass determination by ESI-TOF. Predicted MW is 19412.29118 (+/- 10Da by ESI-TOF). Observed MW is 19412.26.

  • Biological Activity - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799), expandable thumbnail

    Biological Activity - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799)

    Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 10^7 units/mg.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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