Recombinant Human IL-18 Protein (His tag) (Active)
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Recombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with >95%, <0.1 EU/mg endotoxin level, suitable for Mass Spec, SDS-PAGE, HPLC, Cell Culture, FuncS.
View Alternative Names
IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma
- Mass Spec
Lab
Mass Spectrometry - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)
Mass determination by ESI-TOF. Predicted MW is 19412.29118 (+/- 10Da by ESI-TOF). Observed MW is 19412.26.
- Biological Activity
Supplier Data
Biological Activity - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)
Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 10^7 units/mg.
- SDS-PAGE
Lab
SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (AB310799)
SDS-PAGE analysis of ab310799
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Specifications
Form
Lyophilized
Additional notes
SDS-PAGE >= 95%
General info
Function
Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed : 14528293, PubMed : 25500532, PubMed : 37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed : 10653850). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33883744).
Sequence similarities
Belongs to the IL-1 family.
Post-translational modifications
The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form (PubMed:15326478, PubMed:25261253, PubMed:37993712, PubMed:37993714, PubMed:9334240). The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor (PubMed:37993714). Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites (PubMed:37993714). The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744, PubMed:37993714). In contrast, cleavage by CASP3 inactivates IL18 (PubMed:37993714, PubMed:9334240).
Target data
Complementary Products
Product promise
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