Recombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for MS, SDS-PAGE, HPLC, Cell Culture.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.1 EU/mg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- MS, SDS-PAGE, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
M Y F G K L E S K L S V I R N L N D Q V L F I D Q G N R P L F E D M T D S D C R D N A P R T I F I I S M Y K D S Q P R G M A V T I S V K C E K I S T L S C E N K I I S F K E M N P P D N I K D T K S D I I F F Q R S V P G H D N K M Q F E S S S Y E G Y F L A C E K E R D L F K L I L K K E D E L G D R S I M F T V Q N E D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed:10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed:14528293, PubMed:25500532, PubMed:37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed:10653850). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744).
Alternative names
IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma
Recommended products
Recombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for MS, SDS-PAGE, HPLC, Cell Culture.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.1 EU/mg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- MS, SDS-PAGE, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 107 units/mg.
- Accession
- Q14116-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- M Y F G K L E S K L S V I R N L N D Q V L F I D Q G N R P L F E D M T D S D C R D N A P R T I F I I S M Y K D S Q P R G M A V T I S V K C E K I S T L S C E N K I I S F K E M N P P D N I K D T K S D I I F F Q R S V P G H D N K M Q F E S S S Y E G Y F L A C E K E R D L F K L I L K K E D E L G D R S I M F T V Q N E D
- Accession
- Q14116
- Protein length
- Full Length
- Predicted molecular weight
- 19.4 kDa
- Actual molecular weight
- 19.4 kDa
- Amino acids
- 37 to 193
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed:10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed:14528293, PubMed:25500532, PubMed:37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed:10653850). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744).
- Sequence similarities
Belongs to the IL-1 family.
- Post-translational modifications
The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form (PubMed:15326478, PubMed:25261253, PubMed:37993712, PubMed:37993714, PubMed:9334240). The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor (PubMed:37993714). Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites (PubMed:37993714). The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744, PubMed:37993714). In contrast, cleavage by CASP3 inactivates IL18 (PubMed:37993714, PubMed:9334240).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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3 product images
SDS-PAGE - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799)
SDS-PAGE analysis of ab310799
Mass Spectrometry - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799)
Mass determination by ESI-TOF. Predicted MW is 19412.29118 (+/- 10Da by ESI-TOF). Observed MW is 19412.26.
Biological Activity - Recombinant Human IL-18 Protein (His tag) (Active) (ab310799)
Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 10^7 units/mg.
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