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AB283447

Recombinant human IL-1RA protein (Active)

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Recombinant human IL-1RA protein (Active) is a Human Full Length protein, in the 26 to 177 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

IL1F3, IL1RA, IL1RN, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor

4 Images
Functional Studies - Recombinant human IL-1RA protein (Active) (AB283447)
  • FuncS

Supplier Data

Functional Studies - Recombinant human IL-1RA protein (Active) (AB283447)

Fully biologically active determined by its ability to inhibit IL-1 alpha-depedent proliferation in D10.G4.1 mouse helper T cells.

ED50 for this effect is ≤42.41 ng/mL corresponding to a specific activity of 2.36 x 104 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3395756-2

Mass Spectrometry - Recombinant human IL-1RA protein (Active) (AB283447)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human IL-1RA protein (Active) (AB283447)

Mass determination by ESI-TOF.

Predicted MW is 17183.48 Da (+/- 10 Da by ESI-TOF). Observed MW is 17184.72

HPLC - Recombinant human IL-1RA protein (Active) (AB283447)
  • HPLC

Supplier Data

HPLC - Recombinant human IL-1RA protein (Active) (AB283447)

HPLC analysis of ab283447

SDS-PAGE - Recombinant human IL-1RA protein (Active) (AB283447)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IL-1RA protein (Active) (AB283447)

SDS-page analysis of ab283447

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, Cell Culture, SDS-PAGE, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by its ability to inhibit IL-1 alpha-depedent proliferation in D10.G4.1 mouse helper T cells.

ED50 for this effet is ≤42.41 ng/mL corresponding to a specific activity of 2.36 x 104 units/mg.

Accession

P18510

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"RPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDVVPIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDKRFAFIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVTKFYFQEDE","proteinLength":"Full Length","predictedMolecularWeight":"17.18 kDa","actualMolecularWeight":null,"aminoAcidEnd":177,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P18510","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.

Sequence similarities

Belongs to the IL-1 family.

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Product protocols

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Target data

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
See full target information IL1RN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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