Recombinant human IL-2 Receptor alpha protein (Active) is a Human Fragment protein, in the 22 to 213 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
E L C D D D P P E I P H A T F K A M A Y K E G T M L N C E C K R G F R R I K S G S L Y M L C T G N S S H S S W D N Q C Q C T S S A T R N T T K Q V T P Q P E E Q K E R K T T E M Q S P M Q P V D Q A S L P G H C R E P P P W E N E A T E R I Y H F V V G Q M V Y Y Q C V Q G Y R A L H R G P A E S V C K M T H G K T R W T Q P Q L I C T G E M E T S Q F P G E E K P Q A S P E G R P E S E T S C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Alternative names
CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA
Recommended products
Recombinant human IL-2 Receptor alpha protein (Active) is a Human Fragment protein, in the 22 to 213 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind ab221397 with a linear range of 0.01-1.11 μg/mL.
- Accession
- P01589-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- E L C D D D P P E I P H A T F K A M A Y K E G T M L N C E C K R G F R R I K S G S L Y M L C T G N S S H S S W D N Q C Q C T S S A T R N T T K Q V T P Q P E E Q K E R K T T E M Q S P M Q P V D Q A S L P G H C R E P P P W E N E A T E R I Y H F V V G Q M V Y Y Q C V Q G Y R A L H R G P A E S V C K M T H G K T R W T Q P Q L I C T G E M E T S Q F P G E E K P Q A S P E G R P E S E T S C
- Accession
- P01589
- Protein length
- Fragment
- Predicted molecular weight
- 23.6 kDa
- Amino acids
- 22 to 213
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Product promise
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Full details and terms and conditions can be found here:
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5 product images
SDS-PAGE - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)
SDS-PAGE of reduced ab221397 stained overnight with Coomassie Blue. The protein migrates as 34-46 kDa under reducing conditions due to glycosylation.
Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)
Immobilized Biotinylated Human IL-2, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind ab221397 with a linear range of 1-39 ng/mL.
Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)
ab221397 captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200).
Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)
Loaded ab221397 on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 18 nM as determined in BLI assay (ForteBio Octet Red96e).
Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)
ab221397 inhibits the IL-2 dependent proliferatino of Mo7e cells. The EC50 for this effect is 0.35-0.77 μg/mL.
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