Recombinant human IL-22 protein (Active) is a Human Full Length protein, in the 34 to 179 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
A P I S S H C R L D K S N F Q Q P Y I T N R T F M L A K E A S L A D N N T D V R L I G E K L F H G V S M S E R C Y L M K Q V L N F T L E E V L F P Q S D R F Q P Y M Q E V V P F L A R L S N R L S T C H I E G D D L H I Q R N V Q K L K D T V K K L G E S G E I K A I G E L D L L F M S L R N A C I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed:17204547). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed:17204547). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB (PubMed:10875937, PubMed:18599299). Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways (PubMed:25793261, PubMed:31311100). Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (By similarity). Promotes epithelial cell spreading (By similarity).
Alternative names
ILTIF, ZCYTO18, UNQ3099/PRO10096, IL22, Interleukin-22, IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF
Recommended products
Recombinant human IL-22 protein (Active) is a Human Full Length protein, in the 34 to 179 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.
ED50 is 390 pg/ml.
- Accession
- Q9GZX6-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A P I S S H C R L D K S N F Q Q P Y I T N R T F M L A K E A S L A D N N T D V R L I G E K L F H G V S M S E R C Y L M K Q V L N F T L E E V L F P Q S D R F Q P Y M Q E V V P F L A R L S N R L S T C H I E G D D L H I Q R N V Q K L K D T V K K L G E S G E I K A I G E L D L L F M S L R N A C I
- Accession
- Q9GZX6
- Protein length
- Full Length
- Predicted molecular weight
- 16.81 kDa
- Actual molecular weight
- 16.81 kDa
- Amino acids
- 34 to 179
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
=95% Purity by HPLC
General info
- Function
Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed:17204547). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed:17204547). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB (PubMed:10875937, PubMed:18599299). Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways (PubMed:25793261, PubMed:31311100). Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (By similarity). Promotes epithelial cell spreading (By similarity).
- Sequence similarities
Belongs to the IL-10 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant human IL-22 protein (Active) (ab280331)
Functional analysis of ab280331.
Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.
ED50 is 390 pg/ml.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3377996-1
SDS-PAGE - Recombinant human IL-22 protein (Active) (ab280331)
SDS-PAGE analysis of ab280331.
Mass Spectrometry - Recombinant human IL-22 protein (Active) (ab280331)
Mass determination by ESI-TOF.
Predicted MW is 16806.39 Da (+/- 10 Da by ESI-TOF). Observed MW is 16809.33
HPLC - Recombinant human IL-22 protein (Active) (ab280331)
HPLC analysis of ab280331.
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Product protocols
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