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AB256045

Recombinant human IL-25 protein (Active)

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Recombinant human IL-25 protein (Active) is a Human Full Length protein, in the 33 to 177 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

IL17E, UNQ3120/PRO10272, IL25, Interleukin-25, IL-25, Interleukin-17E, IL-17E

1 Images
SDS-PAGE - Recombinant human IL-25 protein (Active) (AB256045)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IL-25 protein (Active) (AB256045)

SDS-PAGE analysis of 1μg ab256045 under reducing (Lane 1) and non-reducing (Lane 2) conditions. ab256045 is a homodimer with a predicted MW of 33.7 kDa (monomer MW is 16.9 kDa).

4-20% Tris-Glycine gel. Coomassie Blue staining.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

IL-8 production in human PBMCs.

Accession

Q9H293

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in 10 mM HCl

Storage buffer

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MYSHWPSCCPSKGQDTSEELLRWSTVPVPPLEPARPNRHPESCRASEDGPLNSRAISPWRYELDRDLNRLPQDLYHARCLCPHCVSLQTGSHMDPRGNSELLYHNQTVFYRRPCHGEKGTHKGYCLERRLYRVSLACVCVRPRVMG","proteinLength":"Full Length","predictedMolecularWeight":"16.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":177,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H293","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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Specifications

Form

Lyophilized

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General info

Function

Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production (PubMed : 15860795, PubMed : 25821217). Promotes and augments T-helper type 2 responses locally or systemically (PubMed : 25821217). Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction (By similarity). In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13 (PubMed : 25821217). Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways (PubMed : 15860795). Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13 (PubMed : 11754819).

Sequence similarities

Belongs to the IL-17 family.

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Product protocols

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Target data

Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production (PubMed : 15860795, PubMed : 25821217). Promotes and augments T-helper type 2 responses locally or systemically (PubMed : 25821217). Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction (By similarity). In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13 (PubMed : 25821217). Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways (PubMed : 15860795). Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13 (PubMed : 11754819).
See full target information IL25
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