Recombinant human IL-28A protein is a Human Full Length protein, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >98% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
P V A R L H G A L P D A R G C H I A Q F K S L S P Q E L Q A F K R A K D A L E E S L L L K D C R C H S R L F P R T W D L R Q L Q V R E R P M A L E A E L A L T L K V L E A T A D T D P A L V D V L D Q P L H T L H H I L S Q F R A C I Q P Q P T A G P R T R G R L H H W L Y R L Q E A P K K E S P G C L E A S V T F N L F R L L T R D L N C V A S G D L C V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
Alternative names
IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A
Recommended products
Recombinant human IL-28A protein is a Human Full Length protein, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >98% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Biological activity of ab50037 is determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells.
- Accession
- Q8IZJ0-1
- Animal free
- No
- Species
- Human
- Concentration
Sequence info
Amino acid sequence
- P V A R L H G A L P D A R G C H I A Q F K S L S P Q E L Q A F K R A K D A L E E S L L L K D C R C H S R L F P R T W D L R Q L Q V R E R P M A L E A E L A L T L K V L E A T A D T D P A L V D V L D Q P L H T L H H I L S Q F R A C I Q P Q P T A G P R T R G R L H H W L Y R L Q E A P K K E S P G C L E A S V T F N L F R L L T R D L N C V A S G D L C V
- Accession
- Q8IZJ0
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).
General info
- Function
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
- Sequence similarities
Belongs to the lambda interferon family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- A few weeks
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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1 product image
Western blot - Recombinant human IL-28A protein (ab50037)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-IL-28 + IL-29 antibody [EPR14608] ab209048 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-IL-28 + IL-29 antibody [EPR14608] (HRP Anti-IL-28 + IL-29 antibody [EPR14608] ab209048) at 1/10000 dilution
All lanes: Western blot - Recombinant human IL-28A protein (ab50037) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 20 kDa
Exposure time: 10s
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