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AB50037

Recombinant human IL-28A protein

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Recombinant human IL-28A protein is a Human Full Length protein, expressed in Escherichia coli, with >98%, suitable for SDS-PAGE, FuncS.

View Alternative Names

IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

1 Images
Western blot - Recombinant human IL-28A protein (AB50037)
  • WB

Lab

Western blot - Recombinant human IL-28A protein (AB50037)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209048 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-IL-28 + IL-29 antibody [EPR14608] (<a href='/en-us/products/primary-antibodies/hrp-il-28-il-29-antibody-epr14608-ab209048'>ab209048</a>) at 1/10000 dilution

All lanes:

Western blot - Recombinant human IL-28A protein (ab50037) at 0.1 µg

Predicted band size: 22 kDa

Observed band size: 20 kDa

true

Exposure time: 10s

Key facts

Purity

>98% SDS-PAGE

Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Biological activity of ab50037 is determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells.

Accession

Animal free

No

Carrier free

No

Species

Human

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Primary Antibodies

AB245076

Anti-IL-28A antibody [EPR20974-48] - BSA and Azide free (Detector)

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Sandwich ELISA - Anti-IL-28A antibody [EPR20974-48] - BSA and Azide free (Detector) (AB245076)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"PVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQVRERPMALEAELALTLKVLEATADTDPALVDVLDQPLHTLHHILSQFRACIQPQPTAGPRTRGRLHHWLYRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8IZJ0","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
A few weeks
Appropriate long-term storage conditions
-20°C
True
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General info

Function

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

Sequence similarities

Belongs to the lambda interferon family.

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Product protocols

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Target data

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL2
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