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AB288800

Recombinant Human IL-28A Protein (Active)

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Recombinant Human IL-28A Protein (Active) is a Human Full Length protein, in the 26 to 200 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, Mass Spec, FuncS.

View Alternative Names

IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

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Biological Activity - Recombinant Human IL-28A Protein (Active) (AB288800)
  • Biological Activity

Lab

Biological Activity - Recombinant Human IL-28A Protein (Active) (AB288800)

Fully biologically active determined by the dose dependant JAK/STAT/ISGF3 pathway activation of human embryonic kidney (HEK)-293 cells overexpressing IFNLR and IL10R inducible SEAP receptor genes. Results obtained with Invivogen HEK-Blue™ IFN-λ cells. ED50 ≤ 0.08 ng/ml, corresponding to a specific activity of 1.25 x 107 units/mg.

Mass Spectrometry - Recombinant Human IL-28A Protein (Active) (AB288800)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human IL-28A Protein (Active) (AB288800)

Mass determination by ESI-TOF. Predicted MW is 19811.95 Da (+/-10 Da by ESI-TOF). Observed MW is 20176.23 Da.

HPLC - Recombinant Human IL-28A Protein (Active) (AB288800)
  • HPLC

Supplier Data

HPLC - Recombinant Human IL-28A Protein (Active) (AB288800)

HPLC analysis of ab288800

SDS-PAGE - Recombinant Human IL-28A Protein (Active) (AB288800)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IL-28A Protein (Active) (AB288800)

SDS-PAGE analysis of ab288800

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, HPLC, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependant JAK/STAT/ISGF3 pathway activation of human embryonic kidney (HEK)-293 cells overexpressing IFNLR and IL10R inducible SEAP receptor genes. Results obtained with InvivoGen HEK-Blue™ IFN-λ cells (Cat code., hkb-ifnl). ED50 ≤ 0.08 ng/ml, corresponding to a specific activity of 1.25 x 10^7 units/mg.

Accession

Q8IZJ0

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"VPVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQVRERPMALEAELALTLKVLEATADTDPALVDVLDQPLHTLHHILSQFRACIQPQPTAGPRTRGRLHHWLYRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV","proteinLength":"Full Length","predictedMolecularWeight":"19.81 kDa","actualMolecularWeight":"19.81 kDa","aminoAcidEnd":200,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8IZJ0","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

SDS-PAGE >= 95%

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General info

Function

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

Sequence similarities

Belongs to the lambda interferon family.

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Product protocols

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Target data

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL2
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