Recombinant Human IL-28A Protein (Active) is a Human Full Length protein, in the 26 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, MS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, MS
- Biologically active
- Yes
Amino acid sequence
V P V A R L H G A L P D A R G C H I A Q F K S L S P Q E L Q A F K R A K D A L E E S L L L K D C R C H S R L F P R T W D L R Q L Q V R E R P M A L E A E L A L T L K V L E A T A D T D P A L V D V L D Q P L H T L H H I L S Q F R A C I Q P Q P T A G P R T R G R L H H W L Y R L Q E A P K K E S P G C L E A S V T F N L F R L L T R D L N C V A S G D L C V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
Alternative names
IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A
Recommended products
Recombinant Human IL-28A Protein (Active) is a Human Full Length protein, in the 26 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, MS
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependant JAK/STAT/ISGF3 pathway activation of human embryonic kidney (HEK)-293 cells overexpressing IFNLR and IL10R inducible SEAP receptor genes. Results obtained with InvivoGen HEK-Blue™ IFN-λ cells (Cat code., hkb-ifnl). ED50 ≤ 0.08 ng/ml, corresponding to a specific activity of 1.25 x 10^7 units/mg.
- Accession
- Q8IZJ0-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- V P V A R L H G A L P D A R G C H I A Q F K S L S P Q E L Q A F K R A K D A L E E S L L L K D C R C H S R L F P R T W D L R Q L Q V R E R P M A L E A E L A L T L K V L E A T A D T D P A L V D V L D Q P L H T L H H I L S Q F R A C I Q P Q P T A G P R T R G R L H H W L Y R L Q E A P K K E S P G C L E A S V T F N L F R L L T R D L N C V A S G D L C V
- Accession
- Q8IZJ0
- Protein length
- Full Length
- Predicted molecular weight
- 19.81 kDa
- Actual molecular weight
- 19.81 kDa
- Amino acids
- 26 to 200
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
- Sequence similarities
Belongs to the lambda interferon family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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4 product images
Biological Activity - Recombinant Human IL-28A Protein (Active) (ab288800)
Fully biologically active determined by the dose dependant JAK/STAT/ISGF3 pathway activation of human embryonic kidney (HEK)-293 cells overexpressing IFNLR and IL10R inducible SEAP receptor genes. Results obtained with Invivogen HEK-Blue™ IFN-λ cells. ED50 ≤ 0.08 ng/ml, corresponding to a specific activity of 1.25 x 107 units/mg.
SDS-PAGE - Recombinant Human IL-28A Protein (Active) (ab288800)
SDS-PAGE analysis of ab288800
Mass Spectrometry - Recombinant Human IL-28A Protein (Active) (ab288800)
Mass determination by ESI-TOF. Predicted MW is 19811.95 Da (+/-10 Da by ESI-TOF). Observed MW is 20176.23 Da.
HPLC - Recombinant Human IL-28A Protein (Active) (ab288800)
HPLC analysis of ab288800
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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